1. Anti-inflammatory agents reduce microglial response, demyelinating process and neuronal toxin uptake in a model of encephalopathy produced by Shiga Toxin 2
- Author
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Jorge Goldstein, Adriana Cangelosi, Myriam Nuñez, Patricia Geoghegan, David Arenas-Mosquera, Alipio Pinto, and Clara Berdasco
- Subjects
0301 basic medicine ,Lipopolysaccharides ,Male ,Anti-Inflammatory Agents ,Ciencias de la Salud ,Striatum ,Pharmacology ,Shiga Toxin 2 ,Dexamethasone ,Etanercept ,Rats, Sprague-Dawley ,Myelin ,Mice ,0302 clinical medicine ,Escherichia coli Infections ,Brain Diseases ,NEUROPROTECTION ,biology ,Microglia ,Shiga toxin ,ENCEPHALOPATHY ,General Medicine ,DEXAMETHASONE ,Oligodendroglia ,Infectious Diseases ,medicine.anatomical_structure ,Enterohemorrhagic Escherichia coli ,purl.org/becyt/ford/3 [https] ,medicine.symptom ,medicine.drug ,Microbiology (medical) ,CIENCIAS MÉDICAS Y DE LA SALUD ,Encephalopathy ,Inflammation ,Microbiology ,Neuroprotection ,purl.org/becyt/ford/3.3 [https] ,03 medical and health sciences ,INFLAMMATION ,medicine ,ETANERCEPT ,Animals ,Humans ,HUS ,medicine.disease ,Rats ,Enfermedades Infecciosas ,030104 developmental biology ,nervous system ,biology.protein ,030217 neurology & neurosurgery - Abstract
Infections by Enterohemorrhagic Escherichia coli may cause in addition to hemolytic uremic syndrome neurological disorders which may lead to fatal outcomes in patients. The brain striatum is usually affected during this outcome. The aim of this study was to determine in this area the role of the microglia in pro-inflammatory events that may occur during Shiga toxin 2 intoxication and consequently to this, whether oligodendrocytes were being affected. In the present paper we demonstrated that anti-inflammatory treatments reduced deleterious effects in brain striatal cells exposed to Shiga toxin 2 and LPS. While dexamethasone treatment decreased microglial activation and recovered myelin integrity in the mice striatum, etanercept treatment decreased neuronal uptake of Stx2 in rat striatal neurons, improving the affected area from toxin-derived injury. In conclusion, microglial activation is related to pro-inflammatory events that may deteriorate the brain function during intoxication with Stx2 and LPS. Consequently, the role of anti-inflammatory agents in the treatment of EHEC-derived encephalopathy should be studied in clinical trials. Fil: Vasconcelos Esteves Pinto, Alipio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Berdasco, Clara Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Arenas Mosquera, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Cangelosi, Adriana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Geoghegan, Patricia A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Nuñez, Myriam C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Goldstein Raij, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
- Published
- 2018