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Your search keyword '"M. Blecher"' showing total 12 results
Start Over Author "M. Blecher" Publisher elsevier inc. on behalf of american society for biochemistry and molecular biology
12 results on '"M. Blecher"'

1. Hormone receptors. 3. Properties of glucagon-binding proteins isolated from liver plasma membranes.

Author

Giorgio NA, Johnson CB, and Blecher M

Subjects
Adenylyl Cyclases metabolism, Adrenocorticotropic Hormone metabolism, Animals, Binding, Competitive, Cell Membrane analysis, Cell Membrane enzymology, Cell Membrane metabolism, Chromatography, Gel, Chromatography, Thin Layer, Detergents pharmacology, Hydrogen-Ion Concentration, Iodides, Iodine Radioisotopes, Liver cytology, Liver enzymology, Molecular Weight, Protein Binding, Rats, Sodium, Solubility, Structure-Activity Relationship, Glucagon metabolism, Liver metabolism, Proteins isolation & purification, Receptors, Cell Surface
Published
1974

2. Dopamine-1-mediated stimulation of phospholipase C activity in rat renal cortical membranes.

Author

Felder CC, Blecher M, and Jose PA

Subjects
Animals, Benzazepines pharmacology, Cell Membrane metabolism, Dopamine Agents pharmacology, Fenoldopam, Inositol metabolism, Inositol Phosphates metabolism, Kinetics, Magnesium pharmacology, Microvilli metabolism, Phentolamine pharmacology, Prazosin pharmacology, Receptors, Dopamine drug effects, Receptors, Dopamine D1, Kidney Cortex metabolism, Phosphatidylinositols metabolism, Receptors, Dopamine physiology, Type C Phospholipases metabolism
Abstract

Phospholipase C (PL-C) mediates transduction of neurotransmitter signals across membranes via hydrolysis of phosphatidylinositol-4,5-bisphosphate (PIP2), leading to generation of second messengers inositol-1,4,5-trisphosphate and diacylglycerol. In this study, dopamine-1 (DA-1) but not dopamine-2 (DA-2) agonists were shown to stimulate PL-C activity in renal cortical membranes. The DA-1 agonist, SKF 82526, stimulated the release of inositol phosphates from renal cortical membranes prelabeled with [3H]myoinositol. The majority of the label (75%) was found in phosphatidylinositol followed by PIP2 (15%) and phosphatidylinositol-4-phosphate (10%). A DA-1 specific effect on PL-C activity was also observed in an in vitro assay of PL-C activity in renal cortical membranes and basolateral and brush border membranes using [3H]PIP2 as the substrate. Dopamine and SKF 82526 stimulated the release of inositol phosphates from added [3H]PIP2 in a concentration-dependent manner. This release was blocked by the DA-1 antagonist SCH 23390 but not by the alpha-adrenergic antagonists phentolamine and prazosin. In contrast, the DA-2 agonist LY 171555 had no effect on inositol phosphate release. Guanosine 5'-(3-O-thio)triphosphate enhanced while guanyl-5'-yl thiophosphate attenuated the DA-1 agonist-stimulated PL-C activity. PL-C activity as measured by [3H]PIP2 hydrolysis had a pH optimum of 6.5, was inhibited by Mg2+ concentrations above 1 mM, was linear with time and protein concentration, and was sensitive to phosphatidylserine and calcium concentrations. We conclude that PL-C is activated by DA-1 but not DA-2 agonists in renal cortical membranes as well as both the basolateral and brush border renal tubular membranes. It is speculated that this action may mediate the natriuretic effects of dopamine in renal tubular epithelia.

Published
1989

7. Controle of the metabolism and lipolytic effects of cyclic 3',5'-adenosine monophosphate in adipose tissue by insulin, methyl xanthines, and nicotinic acid.

Author

Blecher M, Merlino NS, and Ro'Ane JT

Subjects
Animals, Blood Glucose, Cyclic AMP metabolism, Depression, Chemical, Diabetes Mellitus, Experimental metabolism, Epididymis metabolism, Hyperinsulinism metabolism, Kinetics, Lipid Metabolism, Male, Phosphoric Monoester Hydrolases metabolism, Rats, Stimulation, Chemical, Adenine Nucleotides metabolism, Adipose Tissue metabolism, Caffeine pharmacology, Insulin pharmacology, Nicotinic Acids pharmacology, Theophylline pharmacology
Published
1968

9. Metabolism of dibutyryl cyclic adenosine 3',5'-monophosphate during its regulation of lipolysis and glucose oxidation in isolated rat epididymal adipocytes.

Author

Blecher M, Ro'Ane JT, and Flynn PD

Subjects
Adipose Tissue cytology, Adipose Tissue drug effects, Animals, Butyrates metabolism, Cyclic AMP metabolism, Cyclic AMP pharmacology, Depression, Chemical, Epididymis metabolism, Male, Metabolism drug effects, Rats, Stimulation, Chemical, Tritium, Adenine Nucleotides metabolism, Adipose Tissue metabolism, Glucose metabolism, Lipid Metabolism
Published
1970

10. Independence of the effects of epinephrine, glucagon, and adrenocorticotropin on glucose utilization from those on lipolysis in isolated rat adipose cells.

Author

Blecher M, Merlino NS, Ro'Ane JT, and Flynn PD

Subjects
Adenylyl Cyclases metabolism, Adipose Tissue cytology, Adipose Tissue drug effects, Adrenocorticotropic Hormone antagonists & inhibitors, Animals, Binding Sites, Carbon Isotopes, Cyclic AMP biosynthesis, Depression, Chemical, Epididymis, Epinephrine antagonists & inhibitors, Epinephrine pharmacology, Glucagon antagonists & inhibitors, Male, Nicotinic Acids pharmacology, Oxidation-Reduction, Prostaglandins pharmacology, Rats, Sensory Receptor Cells, Stimulation, Chemical, Sympatholytics pharmacology, Theophylline pharmacology, Xanthines pharmacology, Adipose Tissue metabolism, Adrenocorticotropic Hormone pharmacology, Glucagon pharmacology, Glucose metabolism, Lipid Metabolism
Published
1969

12. Enzymatic deacylation of mono- and dibutyryl derivatives of cyclic adenosine 3',5'-monophosphate by extracts of rat tissues.

Author

Blecher M and Hunt NH

Subjects
Adenosine Monophosphate, Adipose Tissue enzymology, Animals, Brain enzymology, Chromatography, Thin Layer, Cytosol enzymology, Cytosol metabolism, Kidney enzymology, Kidney Cortex enzymology, Kidney Medulla enzymology, Liver enzymology, Male, Myocardium enzymology, Rats, Subcellular Fractions enzymology, Tritium, Amidohydrolases metabolism, Butyrates metabolism, Cyclic AMP metabolism, Esterases metabolism, Nucleotidases metabolism, Phosphoric Diester Hydrolases metabolism
Published
1972

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