1. Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice
- Author
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Victoria Wargon, Ana Maria Ornstein, Lautaro Zubeldia-Brenner, Damasia Becu-Villalobos, and Maria Cecilia Ramirez
- Subjects
Male ,medicine.medical_specialty ,LIVER ,CIENCIAS MÉDICAS Y DE LA SALUD ,CYP7B1 ,Cytochrome P450 Family 7 ,SEXUAL DIFFERENCES ,Genética Humana ,Medicina Clínica ,Biology ,Biochemistry ,Mice ,Endocrinology ,Internal medicine ,Endocrinología y Metabolismo ,Gene expression ,medicine ,Animals ,Testosterone ,Promoter Regions, Genetic ,Molecular Biology ,Regulation of gene expression ,Sex Characteristics ,Gene Expression Regulation, Developmental ,Methylation ,DNA Methylation ,Virilism ,GH ,Sexual dimorphism ,Hepatocyte Nuclear Factor 6 ,MICE ,Medicina Básica ,Endocrine disruptor ,Animals, Newborn ,Liver ,CYPS ,Growth Hormone ,DNA methylation ,Steroid Hydroxylases ,Androgens ,Female ,Signal Transduction - Abstract
Neonatal androgenization masculinizes the GH axis and thus may impact on liver gene regulation. Neonatal testosterone administration to female mice decreased (defeminized) female predominant GH-dependent liver gene expression (Hnf6, Adh1, Prlr, Cyp3a41) and did not modify male predominant genes (Cyp7b1, Cyp4a12, Slp). Female predominance of Cis mRNA, an inhibitor of episodic GH signaling pathway, was unaltered. At birth, Cyp7b1 promoter exhibited a higher methylation status in female livers, while the Hnf6 promoter was equally methylated in both sexes; no differences in gene expression were detected at this age. In adulthood, consistent with sex specific predominance, lower methylation status was determined for the Cyp7b1 promoter in males, and for the Hnf6 promoter in females, and this last difference was prevented by neonatal androgenization. Therefore, early steroid treatment or eventually endocrine disruptor exposure may alter methylation status and sexual dimorphic expression of liver genes, and consequently modify liver physiology in females. Fil: Ramirez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Zubeldia Brenner, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Wargon, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Ornstein, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
- Published
- 2013