1. Additional diterpenes from Physcomitrella patens synthesized by copalyl diphosphate/kaurene synthase (PpCPS/KS).
- Author
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Zhan X, Bach SS, Hansen NL, Lunde C, and Simonsen HT
- Subjects
- Gas Chromatography-Mass Spectrometry, Alkyl and Aryl Transferases metabolism, Bryopsida metabolism, Diterpenes metabolism, Organophosphates metabolism, Plant Proteins metabolism
- Abstract
The bifunctional diterpene synthase, copalyl diphosphate/kaurene synthase from the moss Physcomitrella patens (PpCPS/KS), catalyses the formation of at least four diterpenes, including ent-beyerene, ent-sandaracopimaradiene, ent-kaur-16-ene, and 16-hydroxy-ent-kaurene. The enzymatic activity has been confirmed through generation of a targeted PpCPS/KS knock-out mutant in P. patens via homologous recombination, through transient expression of PpCPS/KS in Nicotiana benthamiana, and expression of PpCPS/KS in E. coli. GC-MS analysis of the knock-out mutant shows that it lacks the diterpenoids, supporting that all are products of PpCPS/KS as observed in N. benthamiana and E. coli. These results provide additional knowledge of the mechanism of this bifunctional diterpene synthase, and are in line with proposed reaction mechanisms in kaurene biosynthesis., (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
- Published
- 2015
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