1. Target site mutation and reduced translocation are present in a glyphosate-resistant Lolium multiflorum Lam. biotype from Spain.
- Author
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González-Torralva F, Gil-Humanes J, Barro F, Brants I, and De Prado R
- Subjects
- 3-Phosphoshikimate 1-Carboxyvinyltransferase chemistry, Amino Acid Sequence, Base Sequence, Biological Transport, Glycine pharmacology, Lolium metabolism, Molecular Sequence Data, Proline metabolism, Serine metabolism, Shikimic Acid metabolism, Spain, Species Specificity, Stress, Physiological genetics, Glyphosate, 3-Phosphoshikimate 1-Carboxyvinyltransferase genetics, Adaptation, Physiological genetics, Glycine analogs & derivatives, Herbicides pharmacology, Lolium genetics, Mutation, Plant Leaves metabolism
- Abstract
The resistance mechanism of a glyphosate-resistant Lolium multiflorum Lam. biotype collected in Córdoba (Southern Spain) was examined. Resistance Factor values at three different growth stages ranged between 4.77 and 4.91. At 96 hours after treatment (HAT) the S biotype had accumulated seven times more shikimic acid than the R biotype. There were significant differences in translocation of (14)C-glyphosate between biotypes, i.e. at 96 HAT, the R biotype accumulated in the treated leaf more than 70% of the absorbed herbicide, in comparison with 59.21% of the S biotype; the R biotype translocated only 14.79% of the absorbed (14)C-glyphosate to roots, while in the S population this value was 24.79%. Visualization of (14)C-glyphosate by phosphor imaging showed a reduced distribution in the R biotype compared with the S. Glyphosate metabolism was not involved in the resistance mechanism due to both biotypes showing similar values of glyphosate at 96 HAT. Comparison of the EPSPS gene sequences between biotypes indicated that the R biotype has a proline 182 to serine amino acid substitution. In short, the resistance mechanism of the L. multiflorum Lam. biotype is due to an impaired translocation of the herbicide and an altered target site., (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)
- Published
- 2012
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