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In the pharmaceutical field, a technique to level the surface of powdered medicines is important in the dispensing process because the flatness of the powder surface affects the packaging accuracy directly. This paper investigates a method for leveling of the surface profile of powder using ultrasound vibration on a plate. The system used comprises a V-shaped plate and two ultrasound transducers, and the plate configuration required was determined from the results of simulations performed by finite element analysis. The resonant longitudinal vibration of the transducers generated the resonant flexural vibration mode of the plate at 27.4 kHz, which resulted in transportation and leveling of the powder. The powder was aggregated at the nodal positions of the flexural standing wave, and a correlation was observed between the sound pressure distribution over the plate and the surface profile of the powder. The powder leveling accuracy was investigated by varying the driving phase difference between the transducers, and it was found that a smaller standing wave ratio for the flexural vibration produced higher leveling accuracy. When the input voltage to the transducers was increased, the leveling time decreased and the leveling accuracy improved; the highest leveling accuracy obtained was 2.2 mm at 130 V pp ., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Daisuke Koyama reports financial support was provided by Japan Science and Technology Agency., (Copyright © 2022 Elsevier B.V. All rights reserved.)

This paper investigated an ultrasound vibration cell patterning technique. The ultrasound cell culture dish consisted of a culture dish with a glass bottom and a glass disc with a piezoelectric ring that generated a resonance flexural vibration mode on the bottom of the dish. The growth of HeLa cells on the dish was observed under ultrasound excitation for 24 h. Large ultrasound vibrations on the dish inhibited the cell growth. The acoustic field was predicted with finite element analysis and it was found that the cell growth depended strongly on both the acoustic field in the culture medium and the vibration distribution of the dish. The ultrasound vibrations did not affect the viability of the cells, and the cell growth could be controlled by the flexural vibration of the cultured dish., (Copyright © 2019 Elsevier B.V. All rights reserved.)

As biotechnology develops, techniques for manipulating and separating small particles such as cells and DNA are required in the life sciences. This paper investigates on-chip manipulation of microparticles in small channels by using ultrasonic vibration. The chip consists of a rectangular glass substrate with a cross-shaped channel (cross-section: 2.0×2.0mm 2 ) and four lead zirconate titanate transducers attached to the substrate's four corners. To efficiently generate the flexural vibration mode on the chip, we used finite element analysis to optimize the configurations of the glass substrate and transducers. Silicon carbide microparticles with an average diameter of 50μm were immersed in the channels, which were filled with ethanol. By applying an in-phase input voltage of 75V at 225kHz to the four transducers, a flexural vibration mode with a wavelength of 13mm was excited on the glass substrate, and this flexural vibration generated an acoustic standing wave in the channel. The particles could be trapped at the nodal lines of the standing wave. By controlling the driving phase difference between the two pairs of transducers, the vibrational distribution of the substrate could be moved along the channels so that the acoustic standing wave moved in the same direction. The trapped particles could be manipulated by the two-phase drive, and the transport direction could be switched at the junction of the channels orthogonally by changing the combination of the driving condition to four transducers., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Characteristics of an ultrasonic suction pump that uses a vibrating piston surface and a pipe are numerically simulated and compared with experimental results. Fluid analysis based on the finite-difference time-domain (FDTD) routine is performed, where the nonlinear term and the moving fluid-surface boundary condition are considered. As a result, the suction mechanism of the pump is found to be similar to that of a check valve, where the gap is open during the inflow phase, and it is nearly closed during the outflow phase. The effects of Reynolds number, vibration amplitude and gap thickness on the pump performance are analyzed. The calculated result is in good agreement with the previously measured results., (Copyright © 2016 Elsevier B.V. All rights reserved.)

A technique to form an optical lens array using an ultraviolet (UV)-curable resin and ultrasound was investigated. A UV-curable gel film was formed on a glass plate having four lead zirconate titanate (PZT) transducers. Excitation of the transducers generated a lattice flexural vibration mode on the glass plate. The acoustic radiation force acted to deform the surface of the gel film, so that a lens array could be fabricated on the gel film. The lens array was exposed to UV light under ultrasonication to cure the UV-curable film. The quality factor of the transducer resonance was decreased upon curing of the resin film because the cured resin dampened the vibration of the plate. The acoustic characteristics of the UV-curable gel film were measured by using an ultrasound pulse technique at the MHz range. The sound speed of the gel increased from 987 to 1006 m/s (increase of 1.9%) as the UV exposure time increased. The attenuation coefficient also increased and the larger attenuation of the resin caused the lens array to have a lower quality factor., (Copyright © 2014 Elsevier B.V. All rights reserved.)

The direct finite-difference fluid simulation of acoustic streaming on a fine-meshed three-dimensional model using a graphics processing unit (GPU)-based calculation array is discussed. Airflows are induced by an acoustic traveling wave when an intense sound field is generated in a gap between a bending transducer and a reflector. The calculation results showed good agreement with measurements in a pressure distribution. Several flow vortices were observed near the boundary layer of the reflector and the transducer, which have often been observed near the boundary of acoustic tubes, but have not been observed in previous calculations for this type of ultrasonic air pump., (Copyright © 2014 Elsevier B.V. All rights reserved.)

We developed a plate-shaped non-contact transporter based on ultrasonic vibration, exploiting a phenomenon that a plate can be statically levitated at the place where its gravity and the acoustic radiation force are balanced. In the experiment, four piezoelectric zirconate titanate elements were attached to aluminum plates, on which lattice flexural vibration was excited at 22.3 kHz. The vibrating plates were connected to a loading plate via flexible posts that can minimize the influence of the flexure induced by heavy loads. The distribution of the vibration displacement on the plate was predicted through finite-element analysis to find the appropriate positions of the posts. The maximum levitation height of this transporter was 256 μm with no load. When two vibrating plates were connected to a loading plate, the maximum transportable load was 4.0 kgf., (Copyright © 2013. Published by Elsevier B.V.)

A small-sized sonochemical reactor in which the absolute value of the sound pressure amplitude can be estimated from the vibration velocity of the transducer was investigated. The sound pressure distribution in the reactor and the relationship between the vibration velocity and the sound pressure amplitude were derived through Helmholtz wave equation. The reactor consists of a bolt-clamped Langevin transducer and a rectangular cell with a tungsten reflector. A 3λ/4-standing-wave-field was generated in the reactor to simplify the sound pressure distribution. The sound pressure distribution was measured from the optical refractive index change of water using a laser interferometer. The experimental and theoretical results showed a good agreement in the absolute value of the sound pressure amplitude, and it was confirmed that the sound pressure in the sonochemical reactor can be estimated from the input current of the vibrator., (Copyright © 2012 Elsevier B.V. All rights reserved.)

Artepillin C is the major compound in the Brazilian green propolis from Baccharis dracunculifolia. Our aim in this study was to investigate the anti-inflammatory effects, absorption, and bioavailability of Artepillin C in mice. The animals used were male Swiss mice subjected to: paw oedema by carrageenan (300 microg/paw), carrageenan-induced peritonitis, and prostaglandin E(2) determination. We also measured in vitro nitric oxide production by RAW 264.7 cells and NF-kappaB activity in HEK 293 cells. Finally, we measured the absorption and bioavailability of Artepillin C in plasma from mice by means of GC-MS after a single oral dose (10 mg/kg). In vivo, Artepillin C produced a maximal inhibition of 38% after 360 min on paw oedema. Artepillin C also decreased the number of neutrophils during peritonitis (IC(50): 0.9 (0.5-1.4) mg/kg). Treatment with Artepillin C decreased prostaglandin E(2) by 29+/-3% and 58+/-5% at 1 and 10 mg/kg, respectively, with a mean ID(50) of 8.5 (8.0-8.7) mg/kg). Similarly, in in vitro models, Artepillin C (3, 10, or 100 microM) decreased nitric oxide production by RAW 264.7 cells with a mean IC(50) of 8.5 (7.8-9.2) microM. In HEK 293 cells, Artepillin C reduced NF-kappaB activity with a mean IC(50) of 26 (22-30) mug/ml), suggesting anti-inflammatory activity, particularly during acute inflammation. Lastly, Artepillin C was absorbed after an oral dose (10 mg/kg) with maximal peaks found at 1 h (22 microg/ml). Collectively, Artepillin C showed anti-inflammatory effects mediated, at least in part, by prostaglandin E(2) and nitric oxide inhibition through NF-kappaB modulation, and exhibited bioavailability by oral administration.

We designed and synthesized isoprene analogues of artepillin C, a major component of Brazilian propolis, and investigated the inhibitory activity on lipid peroxidation of rat liver mitochondria (RLM) and RLM toxicity based on isoprenomics. We succeeded in the synthesis of artepillin C isoprene analogues using regioselective prenylation within the range from 22% to 53% total yield. Reactivity of artepillin C and its isoprene analogues with ABTS (2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonate)) radical cations showed only a slight difference among the molecules. The isoprene side-chain elongation analogues of artepillin C showed almost the same inhibitory activity against RLM lipid peroxidation as artepillin C. Artepillin C and its isoprene analogues had very weak RLM uncoupling activity. Moreover, artepillin C and its isoprene analogues exhibited a lower inhibitory activity against adenosine 5'-triphosphate (ATP) synthesis by about two orders of magnitude than the effective inhibitory activity against RLM lipid peroxidation. From these results we conclude that artepillin C isoprene analogues could be potent lipid peroxidation inhibitors having low mitochondrial toxicity. We also conclude that elongation of the isoprene side chain of artepillin C to increase lipophilicity had little influence on the inhibitory activity toward RLM lipid peroxidation.