1. Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM.
- Author
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Kato S, Ohgimoto S, Sharma LB, Kurazono S, Ayata M, Komase K, Takeda M, Takeuchi K, Ihara T, and Ogura H
- Subjects
- Amino Acid Substitution genetics, Animals, Cells, Cultured, Chlorocebus aethiops, Dendritic Cells virology, HeLa Cells, Hemagglutinins, Viral genetics, Humans, Lymphocytes virology, Measles virus genetics, Molecular Sequence Data, Protein Binding, Sequence Analysis, DNA, Signaling Lymphocytic Activation Molecule Family Member 1, Vero Cells, Antigens, CD metabolism, Hemagglutinins, Viral metabolism, Measles virus physiology, Membrane Cofactor Protein metabolism, Receptors, Cell Surface metabolism, Receptors, Virus metabolism, Virus Attachment
- Abstract
The CAM-70 measles virus (MV) vaccine strain is currently used for vaccination against measles. We examined the fusion-inducing ability of the CAM-70 hemagglutinin (H) protein and found that it was impaired in both CD46- and signaling lymphocyte activation molecule (SLAM)-expressing cells. We also generated recombinant MVs possessing H genes derived from the CAM-70 strain. The CAM-70 H protein impaired viral growth in both CD46- and SLAM-expressing cells. In peripheral blood lymphocytes (PBL) and monocyte-derived dendritic cells (Mo-DC), the CAM-70 strain did not grow efficiently. Infection with recombinant MVs revealed that impaired growth of the CAM-70 strain was attributed to the H gene only partly in PBL and largely in Mo-DC. Thus, impaired fusion-inducing ability of the H protein may be one of the underlying molecular mechanisms resulting in the attenuation of the CAM-70 strain.
- Published
- 2009
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