1. BpTCP19 targets BpWRKY53 to negatively regulate jasmonic acid- and dark-induced leaf senescence in Betula platyphylla.
- Author
-
Wang B, Kong WF, Dong W, Su LH, Luan JY, Jiang J, Liu GF, and Li HY
- Subjects
- Transcription Factors metabolism, Transcription Factors genetics, Plant Senescence, Acetates pharmacology, Plants, Genetically Modified, Plant Growth Regulators metabolism, Plant Growth Regulators pharmacology, Cyclopentanes metabolism, Cyclopentanes pharmacology, Oxylipins metabolism, Oxylipins pharmacology, Plant Leaves metabolism, Plant Leaves drug effects, Plant Leaves genetics, Plant Proteins genetics, Plant Proteins metabolism, Darkness, Gene Expression Regulation, Plant drug effects, Betula genetics, Betula metabolism, Betula drug effects
- Abstract
TCP (TEOSINTE-LIKE1, CYCLOIDEA, and PROLIFERATING CELL FACTOR1) is a plant-specific transcription factor that has garnered significant attention due to its wide-ranging involvement in the regulation of plant growth or developmental processes. However, the molecular mechanisms through which TCP genes orchestrate leaf senescence have not been extensively elucidated. BpTCP19, a member of the PCF subfamily in Betula platyphylla, and has high homology to AtTCP19. BpTCP19 displayed pronounced downregulation in response to methyl jasmonate (MeJA) and dark treatment. Overexpressing BpTCP19 in Betula platyphylla led to a delay in leaf senescence, resulting in prolonged leaf greenness under both MeJA and dark conditions. Transcriptome analysis revealed that overexpression of BpTCP19 induced alterations in the expression levels of genes linked to cell proliferation, hormone signaling transduction, and leaf senescence, including the early responsive factor BpWRKY53. Furthermore, through Yeast one-hybrid assays and GUS analysis, BpTCP19 was shown to bind to the promoter region of BpWRKY53, suppressing its expression and thereby retarding leaf senescence. This study elucidates the physiological and molecular functions of BpTCP19 as a central transcriptional regulatory module in leaf senescence and provides a potential target gene for delaying leaf senescence by mitigating sensitivity to external aging signals such as Jasmonic acid (JA) and darkness., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF