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Background: Puumala virus (PUUV), a member of the genus hantavirus, can cause nephropathia epidemica, a mild form of haemorrhagic fever with renal syndrome. The method of choice for the serodiagnosis of hantavirus infections are enzyme-linked immunosorbent assays (ELISAs)., Objectives: Two commercially available PUUV ELISA kits were compared: Hantavirus (Puumala) IgM/IgG ELISA (Progen, Heidelberg, Germany) and PUUMALA IgM and IgG EIA AutoM (Reagena, Toivala, Finland)., Study Design: The sensitivity of the ELISA kits was evaluated with a panel of 55 serum samples from patients with an acute (n=27) or past (n=28) infection based on Progen or Reagena. A panel of 56 serum samples was composed to evaluate the specificity: samples with potentially false positive Progen Puumala IgM results (n=12), seronegative samples for Puumala IgG/IgM with Progen (n=20), and potentially cross reacting samples (n=24). Discrepancies between the two assays were resolved with strip immunoblot. As measure of agreement between Progen and Reagena results, Cohen kappa coefficient was calculated., Results: Reagena showed a higher specificity (IgM 100%, IgG 100%) than Progen Puumala (IgM 73.21%, IgG 100%). However, Reagena showed a slightly lower sensitivity (IgM 96.15%, IgG 97.78%) compared with Progen (IgM 100%, IgG 100%). Substantial agreement with a Cohen kappa of 0.67 and 0.76 was found between the two assays for Puumala IgM and IgG respectively., Conclusions: This study showed a higher specificity of Reagena in comparison to Progen with a lower sensitivity, probably caused by selection bias. In spite of Reagena's lower sensitivity, no acute infection was missed with this assay., (Copyright © 2014 Elsevier B.V. All rights reserved.)