1. Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis and Ureaplasma parvum.
- Author
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Wei HB, Zou SX, Yang XL, Yang DQ, and Chen XD
- Subjects
- Adult, Calibration, Chlamydia Infections microbiology, Chlamydia trachomatis isolation & purification, Cloning, Molecular, DNA Primers, Female, Humans, Plasmids, Sensitivity and Specificity, Sequence Analysis, DNA, Ureaplasma isolation & purification, Ureaplasma Infections microbiology, Urethritis complications, Urethritis microbiology, Chlamydia Infections diagnosis, Chlamydia trachomatis genetics, DNA, Bacterial analysis, Multiplex Polymerase Chain Reaction methods, Ureaplasma genetics, Ureaplasma Infections diagnosis, Urethritis diagnosis
- Abstract
Objectives: Chlamydia trachomatis and Ureaplasma urealyticum are common pathogens of sexually transmitted diseases. The majority of human ureaplasma isolates belong to the new species U. parvum. Clinically, C. trachomatis and U. parvum usually double infect in the nongonococcal urethritis patients. A novel method for simultaneous detection of C. trachomatis and U. parvum was set up in the present work., Design and Methods: Multiple real-time quantitative PCR was developed to allow for rapid, sensitive, specific and quantitative detection of C. trachomatis and U. parvum, simultaneously. To evaluate the applicability of the multiplex real-time quantitative PCR assay to clinical specimens, 64 samples of cervical swabs collected were studied., Results: Compared to the results obtained from single real-time quantitative PCR of C. trachomatis and U. parvum, the specificity, sensitivity and quantitative detection results of multiple real-time quantitative PCR are approximately identical with those of the former., Conclusions: This assay will be of great value in the simultaneous and rapid diagnosis of C. trachomatis and U. parvum in the future., (Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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