1. Cystamine restores GSTA3 levels in Vanin-1 null mice
- Author
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Bruno Maras, Silvestro Duprè, Philippe Naquet, Florent Martin, Luana Di Leandro, M. Eugenia Schininà, Ilias Koutris, Franck Galland, Giuseppina Pitari, Dipartimento di Biologia di Base e Applicata, Università degli Studi dell'Aquila (UNIVAQ), Department of Biochemical Sciences 'Rossi Fanelli', Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Università degli Studi dell'Aquila = University of L'Aquila (UNIVAQ), and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA)
- Subjects
Male ,MESH: Mice, Knockout ,Biochemistry ,Antioxidants ,Mice ,chemistry.chemical_compound ,GSTP1 ,0302 clinical medicine ,pantetheinase ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,glutathione transferase ,cysteamine ,MESH: Animals ,MESH: Superoxide Dismutase ,Chromatography, High Pressure Liquid ,Mice, Knockout ,Mice, Inbred BALB C ,0303 health sciences ,Reverse Transcriptase Polymerase Chain Reaction ,Catalase ,Isoenzymes ,Blot ,Glutathione Reductase ,MESH: Isoenzymes ,MESH: Cell Adhesion Molecules ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Electrophoresis, Polyacrylamide Gel ,Blotting, Western ,MESH: Mice, Inbred BALB C ,Radiation-Protective Agents ,Oxidative phosphorylation ,GPI-Linked Proteins ,Isozyme ,Amidohydrolases ,03 medical and health sciences ,MESH: Radiation-Protective Agents ,Cystamine ,MESH: Catalase ,Physiology (medical) ,cystamine ,MESH: Blotting, Western ,Animals ,MESH: Chromatography, High Pressure Liquid ,MESH: Mice ,030304 developmental biology ,MESH: Glutathione Transferase ,Glutathione Peroxidase ,Superoxide Dismutase ,MESH: Antioxidants ,MESH: Cystamine ,Glutathione ,Molecular biology ,MESH: Male ,chemistry ,Pantetheinase ,vanin-1 ,MESH: Glutathione Peroxidase ,Cysteamine ,Cell Adhesion Molecules ,MESH: Glutathione Reductase ,030217 neurology & neurosurgery ,MESH: Electrophoresis, Polyacrylamide Gel - Abstract
International audience; Free cysteamine levels in mouse tissues have been strictly correlated to the presence of membrane-bound pantetheinase activity encoded by Vanin-1. Vanin-1 is involved in many biological processes in mouse, from thymus homing to sexual development. Vanin-1 -/- mice are fertile and grow and develop normally; they better control inflammation and most of the knockout effects were rescued by cystamine treatment. Gene structure analysis showed the presence of an oxidative stimuli-responsive ARE-like sequence in the promoter. In this paper we investigate antioxidant-detoxifying enzymatic activities at the tissue level, comparing Vanin-1 -/- and wild-type mice. In Vanin-1 null animals we pointed out a decrease in the Se-independent glutathione peroxidase activity. The decrease in enzymatic activity appeared to be correlated to an impairment of GST isoenzyme levels. In particular a significant drop in GSTA3 together with a minor decrement in GSTM1 and an increase in GSTP1 levels was detected in Vanin-1 -/- livers. Cystamine administration to Vanin-1 -/- mice restored specifically GSTA3 levels and the corresponding enzymatic activity without influencing protein expression. A possible role of cystamine on protein stability/folding can be postulated.
- Published
- 2008
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