1. Phylogenetic relationship of psychoactive fungi based on rRNA gene for a large subunit and their identification using the TaqMan assay (II).
- Author
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Maruyama T, Kawahara N, Yokoyama K, Makino Y, Fukiharu T, and Goda Y
- Subjects
- Agaricales chemistry, Agaricales genetics, Base Sequence, DNA, Fungal chemistry, Drug and Narcotic Control, Gas Chromatography-Mass Spectrometry, Hallucinogens analysis, Humans, Japan, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction methods, Psilocybin analysis, RNA, Fungal genetics, Substance Abuse Detection methods, Substance-Related Disorders diagnosis, Agaricales classification, Agaricales isolation & purification, Genes, rRNA genetics
- Abstract
"Magic mushroom (MM)" is the name most commonly given to psychoactive fungi containing the hallucinogenic components: psilocin (1) and psilocybin (2). We investigated the rRNA gene (internal transcribed spacer (ITS) and large subunit (LSU)) of two Panaeolus species and four Psilocybe species fungi (of these, two are non-psilocybin species). On the basis of sequence alignment, we improved the identification system developed in our previous study. In this paper, we describe the new system capable of distinguishing MMs from non-psilocybin Psilocybe species, its application data and the phylogeny of MM species.
- Published
- 2006
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