1. IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis.
- Author
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Manna PP, Hira SK, Das AA, Bandyopadhyay S, and Gupta KK
- Subjects
- Animals, Aorta immunology, Cells, Cultured, Cytotoxicity, Immunologic immunology, Granzymes metabolism, Humans, Interferon-gamma pharmacology, Interleukin-15 metabolism, Killer Cells, Natural immunology, Lipopolysaccharide Receptors, Lymphocyte Activation immunology, Membrane Potential, Mitochondrial, Monocytes metabolism, Swine, T-Lymphocytes, Cytotoxic immunology, Transplantation, Heterologous immunology, Transplantation, Homologous immunology, Tumor Necrosis Factor-alpha pharmacology, Apoptosis immunology, Dendritic Cells immunology, Endothelial Cells immunology, Interleukin-15 immunology
- Abstract
IL-15 is a pleotropic cytokine, which plays an important role in natural killer (NK) cell activity, T cell proliferation, and T cell cytotoxic activity. Dendritic cells (DCs) are the major antigen presenting cells in the immune system and presumed to play an important role in immune recognition of allo and xenotransplantation. We showed that IL-15 activated human peripheral blood DC is cytotoxic to human and porcine aortic endothelial cells. Unlike DCs, CD14+ monocytes show no cytotoxicity against the endothelial cells. This cytotoxic potential of IL-15 activated DC against endothelial cells is dose dependent and increases significantly upon treatment of endothelial cells with inflammatory cytokines like TNF-α or IFN-γ. The cytotoxic potential of IL-15 activated DC is associated with apoptosis of endothelial cells, as indicated by the increased Annexin V staining, caspase activation and loss of mitochondrial membrane potential. Further it was observed that DC mediated cytotoxicity against endothelial cell is mediated via granzyme B possibly secreted by the activated DCs., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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