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Bathing in natural waters is a highly demanded recreational activity in tropical countries because of climatic conditions and availability of bathing sites; but, do users know the water quality of these sites? We determined the physicochemical and microbiological water quality of a highly used bathing site in southern Ecuador. We assessed how bather attendance, rainfall events, and pool location alters the recreational water quality (RWQ). Most of the parameters measured in the stream pools did not accomplish the Ecuadorian and international regulations for recreational water quality. Microbiological water quality diminishes from upstream to downstream pools because of human activities and bathing intensity having potential effects on bather health and eco-touristic development. We found that an increase of bathers is strongly associated with a growing concentration of Escherichia coli. It is suggested better land-use practices and review thoroughly the Ecuadorian regulation to assure a healthy RWQ. Further efforts are needed to identify more risky bathing sites, determine pollution sources, and establish a long-term monitoring program to support the touristic development in countries looking for diversifying their economy., (Copyright © 2019 Elsevier Ltd. All rights reserved.)