1. A novel neuropeptide cellular mechanism in amphibian interrenal steroidogenesis.
- Author
-
Gobbetti A and Zerani M
- Subjects
- 1-Methyl-3-isobutylxanthine pharmacology, Animals, Aromatase metabolism, Aspirin pharmacology, Bucladesine pharmacology, Colforsin pharmacology, Dinoprost metabolism, Dinoprost pharmacology, Dinoprostone metabolism, Female, Hydroxyprostaglandin Dehydrogenases metabolism, In Vitro Techniques, Interrenal Gland drug effects, Interrenal Gland metabolism, Models, Biological, Palmitic Acid, Palmitic Acids pharmacology, Rana esculenta, Estradiol metabolism, Gonadotropin-Releasing Hormone pharmacology, Interrenal Gland physiology, Testosterone metabolism
- Abstract
Interrenals of female Rana esculenta were incubated with gonadotropin-releasing hormone (GnRH), 9-ketoreductase inhibitor (palmitic acid), acetyl salicyclic acid, prostaglandin F2 alpha (PGF2 alpha), forskolin, isobutylmethyl xanthine (IBMX), dibutyril cyclic adenosine monophosphate (dbcAMP). Prostaglandin E2 (PGE2), PGF2 alpha, testosterone and 17 beta-estradiol were assessed on the incubation media. In addition, in the same interrenals, 9-ketoreductase and aromatase activities were evaluated. GnRH increased PGF2 alpha, 17 beta-estradiol, 9-ketoreductase and aromatase, and decreased PGE2 and testosterone. PGF2 alpha increased 17 beta-estradiol and aromatase, and decreased testosterone. Palmitic acid counteracted GnRH effects, while forskolin, IBMX and dbcAMP showed the same PGF2 alpha effects. These results suggest that GnRH stimulates 9-ketoreductase enhancing PGF2 alpha which in turn activates aromatase through cAMP mediation in the interrenal of Rana esculenta.
- Published
- 1995
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