1. Local levels of interleukin-1beta, -4, -6 and tumor necrosis factor alpha in an experimental model of murine osteomyelitis due to staphylococcus aureus.
- Author
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Yoshii T, Magara S, Miyai D, Nishimura H, Kuroki E, Furudoi S, Komori T, and Ohbayashi C
- Subjects
- Animals, Bone and Bones ultrastructure, Female, Mice, Mice, Inbred ICR, Osteoclasts metabolism, Osteomyelitis etiology, Staphylococcal Infections complications, Staphylococcal Infections metabolism, Bone and Bones metabolism, Interleukin-1 metabolism, Interleukin-4 metabolism, Interleukin-6 metabolism, Osteomyelitis metabolism, Osteomyelitis microbiology, Staphylococcus aureus, Tumor Necrosis Factor-alpha metabolism
- Abstract
The purpose of this study is to evaluate local levels of interleukin-1 beta (IL-1 beta), -4 (IL-4), -6 (IL-6), and tumour necrosis factor-alpha (TNF-alpha), in a model of murine osteomyelitis due to Staphylococcus aureus. Cytokine levels in supernatants derived from bone homogenates were determined by enzyme-linked immunosorbent assay, for 28 days following the direct implantation of murine tibiae with S.aureus. Levels of IL-1 beta and IL-6 in infected bone were elevated in the early post-infection period and then decreased. In contrast, TNF-alpha levels remained elevated 3 to 28 days post-infection, while IL-4 levels were elevated late in the course of infection. The histopathology of infected bone showed predominant infiltration of inflammatory cells and bone resorption 3 to 7 days after infection, and bone resorption and adjacent areas of formation 14 to 28 days after infection. These results suggest that the elevated IL-1 beta and IL-6 levels induced by infection may be related to bone damage mainly in the early phase of infection, and that TNF-alpha and IL-4 may at least in part be associated with histopathological changes, including both bone resorption and formation in the later phase of this osteomyelitis model.
- Published
- 2002
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