1. Development of a monoclonal antibody against swine leukocyte antigen (SLA)-DR α chain and evaluation of SLA-DR expression in bone marrow-derived dendritic cells after PRRSV infection.
- Author
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Zhai T, Wu C, Wang N, Shi B, Li J, Chen R, Dong J, Zhang Y, Zhou EM, and Nan Y
- Subjects
- Animals, Blotting, Western, Bone Marrow immunology, Bone Marrow metabolism, Cell Line, Dendritic Cells immunology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Antibody Technique, HEK293 Cells, HLA-DR alpha-Chains metabolism, Humans, Immunoprecipitation, Mice, Mice, Inbred BALB C immunology, Recombinant Proteins, Swine immunology, Antibodies, Monoclonal immunology, Dendritic Cells metabolism, HLA-DR alpha-Chains immunology, Porcine Reproductive and Respiratory Syndrome immunology, Porcine respiratory and reproductive syndrome virus immunology
- Abstract
Porcine reproductive and respiratory syndrome (PRRS) is one of the most common diseases in the global swine industry. PRRSV infection is highly restricted to cells of the monocyte-macrophage lineage. However, the lack of antibodies to swine monocyte-macrophage lineage markers significantly hampers PRRSV research. In this study, we have developed a monoclonal antibody against the swine leukocyte antigen (SLA)-DRα chain and confirmed its reactivity with endogenous expressed SLA-DR in a variety of cell lines and primary swine antigen-presenting cells (PAMs, PBMC and BM-DCs). Moreover, the level of SLA-DR expression after PRRSV infection were evaluated by our homemade Mab and a commercial anti-SLA-DR antibody. Based on our result, the protein level of SLA-DRα expression is increased after PRRSV infection in DC, while the mRNA of both SLA-DRα and SLA-DRβ were significantly inhibited by PRRSV replication. In conclusion, we successfully developed a MAb reactive with endogenous SLA-DR in western blotting, and this MAb could be a useful tool for further research and analysis. Moreover, the inconsistency of SLA-DR expression between protein and mRNA levels may suggest a novel role of DC played during the immune response after PRRSV infection., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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