1. Recombinant canine IL-13 receptor alpha2-Fc fusion protein inhibits canine allergen-specific-IgE production in vitro by peripheral blood mononuclear cells from allergic dogs.
- Author
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Tang L, Boroughs KL, Morales T, Stedman K, Sellins K, Clarke K, McDermott M, Yang S, and McCall C
- Subjects
- Amino Acid Sequence, Animals, Dog Diseases blood, Dogs, Enzyme-Linked Immunosorbent Assay veterinary, Escherichia coli genetics, Humans, Hypersensitivity blood, Hypersensitivity immunology, Immunoglobulin E blood, Immunoglobulin Fc Fragments chemistry, Immunoglobulin Fc Fragments genetics, Immunoglobulin Heavy Chains genetics, Immunoglobulin Heavy Chains immunology, Interleukin-13 chemistry, Interleukin-13 immunology, Interleukin-13 Receptor alpha1 Subunit, Leukocytes, Mononuclear metabolism, Molecular Sequence Data, Receptors, Interleukin genetics, Receptors, Interleukin-13, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Sequence Analysis, DNA, Siphonaptera immunology, Tumor Cells, Cultured, Dog Diseases immunology, Hypersensitivity veterinary, Immunoglobulin E biosynthesis, Immunoglobulin Fc Fragments immunology, Interleukin-13 antagonists & inhibitors, Leukocytes, Mononuclear immunology, Receptors, Interleukin immunology, Recombinant Fusion Proteins immunology
- Abstract
Human IL-13, like IL-4, is involved in the regulation of B-cell development, IgE synthesis and allergic responses. However, because IL-13 does not affect either murine Ig class switching or IgE production in vitro, the use of murine models to study the role of IL-13 in IgE-mediated diseases has been limited. In this communication, we report that recombinant protein of canine IL-13 (rcaIL-13) stimulates production of allergen-specific-IgE in vitro by peripheral blood mononuclear cells (PBMC) from flea allergen-sensitized dogs, and that this stimulation activity is specifically inhibited by recombinant protein of canine IL-13Ralpha2 and Fc fragment of canine IgG heavy chain (rcaIL-13Ralpha2-Fc). The data suggest that the regulatory effects of IL-13 on IgE production in canine PBMC are similar to those reported in humans. Thus, canine IL-13 may be a central mediator of allergic diseases in dogs, and allergic dogs may be excellent models for research on IgE-mediated diseases in humans.
- Published
- 2001
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