1. Phenolic-rich fraction from Rhus verniciflua Stokes (RVS) suppress inflammatory response via NF-kappaB and JNK pathway in lipopolysaccharide-induced RAW 264.7 macrophages.
- Author
-
Jung CH, Kim JH, Hong MH, Seog HM, Oh SH, Lee PJ, Kim GJ, Kim HM, Um JY, and Ko SG
- Subjects
- Animals, Anti-Inflammatory Agents administration & dosage, Anti-Inflammatory Agents chemistry, Cell Line, Cell Survival, Cyclooxygenase 2 drug effects, Dinoprostone metabolism, Dose-Response Relationship, Drug, Gene Expression Regulation drug effects, JNK Mitogen-Activated Protein Kinases metabolism, Lipopolysaccharides, Macrophages drug effects, Macrophages metabolism, Mice, NF-kappa B metabolism, Nitric Oxide metabolism, Nitric Oxide Synthase Type II drug effects, Phenols administration & dosage, Phenols isolation & purification, Phytotherapy, Plant Bark, Plant Extracts chemistry, Plant Extracts pharmacology, Plants, Medicinal, Signal Transduction drug effects, Tumor Necrosis Factor-alpha drug effects, Tumor Necrosis Factor-alpha metabolism, Anti-Inflammatory Agents pharmacology, JNK Mitogen-Activated Protein Kinases drug effects, NF-kappa B drug effects, Phenols pharmacology, Rhus chemistry
- Abstract
The effects of phenolic-rich fraction (PRF) from Rhus verniciflua Stokes (Anacardiaceae) on the activities of cellular signaling molecules that mediate inflammatory responses in LPS-induced RAW 264.7 macrophages were investigated. At various concentrations of PRF significantly inhibited NO, PGE(2) and TNF-alpha production in LPS-induced RAW 264.7 macrophage cells. The PRF also significantly inhibited iNOS and COX-2 protein expression in LPS-induced RAW 264.7 macrophage in a concentration-dependent manner. Transcription factor NF-kappaB plays a key role for the inducible expression of genes mediating proinflammatory effects and here, we show that PRF can inhibit the induction of NF-kappaB activity. The PRF effectively inhibited the iNOS and COX-2 protein expression through suppression of phospho-JNK1/2 activation. Study using PDA HPLC has found that the PRF contains several low molecular compounds (i.e. p-coumaric acid, fustin, kaempferol-3-O-glucoside, sulfuretin, butein, kaempferol). Our results indicate that the anti-inflammatory properties of PRF might result from the inhibition of pro-inflammatory mediators (e.g., NO, PGE(2) and TNF-alpha) by suppression of such signaling pathways as NF-kappaB and JNK1/2.
- Published
- 2007
- Full Text
- View/download PDF