1. Cdc48 and Ufd3, new partners of the ubiquitin protease Ubp3, are required for ribophagy
- Author
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Catherine Dargemont, François Delalande, Mélanie Bonizec, Mickael M. Cohen, Batool Ossareh-Nazari, Svetlana Dokudovskaya, Christine Schaeffer, Alain Van Dorsselaer, Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), and Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Proteasome Endopeptidase Complex ,Saccharomyces cerevisiae Proteins ,Recombinant Fusion Proteins ,medicine.medical_treatment ,Saccharomyces cerevisiae ,Cell Cycle Proteins ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Ubiquitin ,Valosin Containing Protein ,Endopeptidases ,Genetics ,medicine ,Cell Cycle Protein ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,Adaptor Proteins, Signal Transducing ,030304 developmental biology ,Adenosine Triphosphatases ,0303 health sciences ,Protease ,biology ,Scientific Reports ,biology.organism_classification ,Yeast ,Ubiquitin ligase ,Cell biology ,Proteasome ,biology.protein ,Ribosomes ,030217 neurology & neurosurgery ,Deubiquitination - Abstract
Ubiquitin-dependent processes can be antagonized by substrate-specific deubiquitination enzymes involved in many cellular functions. In this study, we show that the yeast Ubp3-Bre5 deubiquitination complex interacts with both the chaperone-like Cdc48, a major actor of the ubiquitin and proteasome system, and Ufd3, a ubiquitin-binding cofactor of Cdc48. We observed that these partners are required for the Ubp3-Bre5-dependent and starvation-induced selective degradation of yeast mature ribosomes, also called ribophagy. By contrast, proteasome-dependent degradation does not participate in this process. Our data favour the idea that these factors cooperate to recognize and deubiquitinate specific substrates of ribophagy before their vacuolar degradation.
- Published
- 2010