1. <scp>OST</scp> 1‐mediated <scp>BTF</scp> 3L phosphorylation positively regulates <scp>CBF</scp> s during plant cold responses
- Author
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Shuhua Yang, Yiting Shi, Yuxin Jia, Xiaoyan Zhang, Chun-Peng Song, Zhizhong Gong, and Yanglin Ding
- Subjects
0301 basic medicine ,Plant growth ,Arabidopsis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Stress, Physiological ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Gene ,Transcription factor ,Freezing tolerance ,General Immunology and Microbiology ,Arabidopsis Proteins ,General Neuroscience ,Articles ,biology.organism_classification ,Cell biology ,Cold Temperature ,030104 developmental biology ,Protein Kinases ,Function (biology) ,Transcription Factors - Abstract
Cold stress is a major environmental factor that negatively affects plant growth and survival. OST1 has been identified as a key protein kinase in plant response to cold stress; however, little is known about the underlying molecular mechanism. In this study, we identified BTF3 and BTF3L (BTF3‐like), β‐subunits of a nascent polypeptide‐associated complex (NAC), as OST1 substrates that positively regulate freezing tolerance. OST1 phosphorylates BTF3 and BTF3L in vitro and in vivo , and facilitates their interaction with C‐repeat‐binding factors (CBFs) to promote CBF stability under cold stress. The phosphorylation of BTF3L at the Ser50 residue by OST1 is required for its function in regulating freezing tolerance. In addition, BTF3 and BTF3L proteins positively regulate the expression of CBF genes. These findings unravel a molecular mechanism by which OST1‐BTF3‐CBF module regulates plant response to cold stress.
- Published
- 2018