1. Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots
- Author
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Isabel Betegón-Putze, Josep Vilarrasa-Blasi, Maria Mar Marquès-Bueno, Yvonne Stahl, Ainoa Planas-Riverola, Rosangela Sozzani, Cristina Martínez, Marta Ibañes, David Frigola, Salomé Prat, Josep Mercadal, Rebecca C. Burkart, Nadja Bosch, Ana Conesa, Ana I. Caño-Delgado, Ministerio de Economía y Competitividad (España), European Research Council, European Commission, Ministerio de Educación, Cultura y Deporte (España), Generalitat de Catalunya, and German Research Foundation
- Subjects
Plant stem cell ,Medicine (General) ,animal structures ,Cell division ,root growth ,QH301-705.5 ,WOX5 ,quiescent centre ,Meristem ,Arabidopsis ,Transcription factor complex ,Plant Biology ,Plant Roots ,Chromatin, Epigenetics, Genomics & Functional Genomics ,General Biochemistry, Genetics and Molecular Biology ,Article ,03 medical and health sciences ,0302 clinical medicine ,R5-920 ,Gene Expression Regulation, Plant ,Gene expression ,Nitriles ,Transcriptional regulation ,mathematical modelling ,Biology (General) ,Transcription factor ,030304 developmental biology ,Homeodomain Proteins ,0303 health sciences ,General Immunology and Microbiology ,biology ,Arabidopsis Proteins ,Applied Mathematics ,Articles ,biology.organism_classification ,Cell biology ,Computational Theory and Mathematics ,BRAVO ,Stem cell ,General Agricultural and Biological Sciences ,Development & Differentiation ,030217 neurology & neurosurgery ,Cell Division ,Information Systems - Abstract
Understanding stem cell regulatory circuits is the next challenge in plant biology, as these cells are essential for tissue growth and organ regeneration in response to stress. In the Arabidopsis primary root apex, stem cell-specific transcription factors BRAVO and WOX5 co-localize in the quiescent centre (QC) cells, where they commonly repress cell division so that these cells can act as a reservoir to replenish surrounding stem cells, yet their molecular connection remains unknown. Genetic and biochemical analysis indicates that BRAVO and WOX5 form a transcription factor complex that modulates gene expression in the QC cells to preserve overall root growth and architecture. Furthermore, by using mathematical modelling we establish that BRAVO uses the WOX5/BRAVO complex to promote WOX5 activity in the stem cells. Our results unveil the importance of transcriptional regulatory circuits in plant stem cell development., A.I.C-D. is a recipient of a BIO2016-78955 grant from the Spanish Ministry of Economy and Competitiveness and a European Research Council, ERC Consolidator Grant (ERC-2015-CoG – 683163). I.B-P. is funded by the FPU15/02822 grant from the Spanish Ministry of Education, Culture and Sport; N.B. by the FI-DGR 2016FI_B 00472 grant from the AGAUR, Generalitat de Catalunya; and A.P-R. by the SEV-2015-0533 from the Severo Ochoa Programme for Centers of Excellence in R&D. M.I. and J.M. acknowledge support from the Spanish Ministry of Economy and Competitiveness and FEDER (EU) through grant FIS2015-66503-C3-3-P, from Ministerio de Ciencia, Innovación y Universidades / Agencia Estatal de Investigación / Fondo Europeo de Desarrollo Regional, Unión Europea through grant PGC2018-101896-B-I00 and from the Generalitat de Catalunya through Grup de Recerca Consolidat 2014 SGR 878 and 2017 SGR 1061. J.M. is funded by the Spanish Ministry of Education through BES-2016-078218. Y.S. and R.C.D. were funded by the Deutsche Forschungsgesellschaft (DFG) (grant STA12/12 1-1). A.I.C.-D.–A.C. collaboration was funded by the European Regional Development Funds and Marie Curie IRSES Project DEANN (PIRSES-GA-2013-612583). CRAG is funded by “Severo Ochoa Programme” from Centers of Excellence in R&D 2016-2019 (SEV-2015-485 0533).
- Published
- 2021