1. Genomic Instability in Peripheral Blood and Buccal Mucosal Cells of Marijuana Smokers: The Impact of Tobacco Smoke
- Author
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Samuel Rangel Claudio, Daniel Araki Ribeiro, Rogerio Correa Peres, Kevin Pereira Marangoni, Daniel Vitor de Souza, and Camila Lima Feitosa Da Silva
- Subjects
Adult ,Male ,0301 basic medicine ,Genome instability ,Karyolysis ,Physiology ,Genomic Instability ,Tobacco smoke ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Smoke ,mental disorders ,Humans ,Cytotoxic T cell ,Medicine ,Oral mucosa cells ,Cannabis ,Blood Cells ,business.industry ,Smoking ,Mouth Mucosa ,General Medicine ,Buccal administration ,peripheral blood ,Prognosis ,Peripheral blood ,Comet assay ,micronucleus test ,030104 developmental biology ,Case-Control Studies ,030220 oncology & carcinogenesis ,Micronucleus test ,Female ,business ,marijuana ,tobacco smoke ,Research Article ,Follow-Up Studies - Abstract
Background The aim of this study was to evaluate cytotoxic, mutagenic and genotoxic effects on buccal mucosa and peripheral blood cells from marijuana and tobacco smokers. Methods For this purpose, a total of 45 volunteers were distributed into four groups: CTRL group (control): individuals who did not smoke marijuana or tobacco (n = 11); Group M: Marijuana smokers (n = 13); Group T: Tobacco smokers (n = 13); Group M + T: Smokers of both marijuana and tobacco (n = 08). Results Smokers of both marijuana and tobacco led an increase of micronucleated cells on buccal mucosa when compared to control group. The occurrence of karyolysis showed significant changes in this group as well. The comet assay data revealed genetic damage in peripheral blood cells for all groups of smokers. Conclusion In summary, our results showed that marijuana and /or tobacco are able to induce genetic damage and cytotoxicity in oral and peripheral blood cells.
- Published
- 2020
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