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Start Over Descriptor "Coagulative necrosis" Publisher escholarship, university of california
3 results on '"Coagulative necrosis"'

1. Histologic changes associated with talaporfin sodium‐mediated photodynamic therapy in rat skin

Author

Moy, Wesley J, Yao, Jonathan, de Feraudy, Sébastien M, White, Sean M, Salvador, Jocelynda, Kelly, Kristen M, and Choi, Bernard

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Cancer, Animals, Lasers, Semiconductor, Male, Photochemotherapy, Photosensitizing Agents, Porphyrins, Port-Wine Stain, Rats, Rats, Sprague-Dawley, Skin, coagulative necrosis, depth of photo-injury, NPe6, photochemical, port-wine stains, Dermatology & Venereal Diseases, Clinical sciences, Dentistry
Abstract

Background and objectiveAlternative treatments are needed to achieve consistent and more complete port wine stain (PWS) removal, especially in darker skin types; photodynamic therapy (PDT) is a promising alternative treatment. To this end, we previously reported on Talaporfin Sodium (TS)-mediated PDT. It is essential to understand treatment tissue effects to design a protocol that will achieve selective vascular injury without ulceration and scarring. The objective of this work is to assess skin changes associated with TS-mediated PDT with clinically relevant treatment parameters.Study design/materials and methodsWe performed TS (0.75 mg/kg)-mediated PDT (664 nm) on Sprague Dawley rats. Radiant exposures were varied between 15 and 100 J/cm2 . We took skin biopsies from subjects at 9 hours following PDT. We assessed the degree and depth of vascular and surrounding tissue injury using histology and immunohistochemical staining.ResultsTS-mediated PDT at 0.75 mg/kg combined with 15 and 25 J/cm2 light doses resulted in vascular injury with minimal epidermal damage. At light dose of 50 J/cm2 , epidermal damage was noted with vascular injury. At light doses >50 J/cm2 , both vascular and surrounding tissue injury were observed in the forms of vasculitis, extravasated red blood cells, and coagulative necrosis. Extensive coagulative necrosis involving deeper adnexal structures was observed for 75 and 100 J/cm2 light doses. Observed depth of injury increased with increasing radiant exposure, although this relationship was not linear.ConclusionTS-mediated PDT can cause selective vascular injury; however, at higher light doses, significant extra-vascular injury was observed. This information can be used to contribute to design of safe protocols to be used for treatment of cutaneous vascular lesions. Lasers Surg. Med. 49:767-772, 2017. © 2017 Wiley Periodicals, Inc.

Published
2017

2. Histologic changes associated with talaporfin sodium-mediated photodynamic therapy in rat skin.

Author

Moy, Wesley, Moy, Wesley, Yao, Jonathan, de Feraudy, Sébastien, White, Sean, Salvador, Jocelynda, Choi, Bernard, Kelly, Kristen, Moy, Wesley, Moy, Wesley, Yao, Jonathan, de Feraudy, Sébastien, White, Sean, Salvador, Jocelynda, Choi, Bernard, and Kelly, Kristen

Abstract

BACKGROUND AND OBJECTIVE: Alternative treatments are needed to achieve consistent and more complete port wine stain (PWS) removal, especially in darker skin types; photodynamic therapy (PDT) is a promising alternative treatment. To this end, we previously reported on Talaporfin Sodium (TS)-mediated PDT. It is essential to understand treatment tissue effects to design a protocol that will achieve selective vascular injury without ulceration and scarring. The objective of this work is to assess skin changes associated with TS-mediated PDT with clinically relevant treatment parameters. STUDY DESIGN/MATERIALS AND METHODS: We performed TS (0.75 mg/kg)-mediated PDT (664 nm) on Sprague Dawley rats. Radiant exposures were varied between 15 and 100 J/cm2 . We took skin biopsies from subjects at 9 hours following PDT. We assessed the degree and depth of vascular and surrounding tissue injury using histology and immunohistochemical staining. RESULTS: TS-mediated PDT at 0.75 mg/kg combined with 15 and 25 J/cm2 light doses resulted in vascular injury with minimal epidermal damage. At light dose of 50 J/cm2 , epidermal damage was noted with vascular injury. At light doses >50 J/cm2 , both vascular and surrounding tissue injury were observed in the forms of vasculitis, extravasated red blood cells, and coagulative necrosis. Extensive coagulative necrosis involving deeper adnexal structures was observed for 75 and 100 J/cm2 light doses. Observed depth of injury increased with increasing radiant exposure, although this relationship was not linear. CONCLUSION: TS-mediated PDT can cause selective vascular injury; however, at higher light doses, significant extra-vascular injury was observed. This information can be used to contribute to design of safe protocols to be used for treatment of cutaneous vascular lesions. Lasers Surg. Med. 49:767-772, 2017. © 2017 Wiley Periodicals, Inc.

Published
2017

3. Restriction-Spectrum Imaging of Bevacizumab-Related Necrosis in a Patient with GBM

Author

Nikdokht eFarid, Daniela Batista Almeida-Freitas, Nathan eWhite, Carrie eMcDonald, Karra eMuller, Scott eVandenBerg, Santosh eKesari, and Anders M. Dale

Subjects
diffusion-weighted imaging (DWI), Cancer Research, medicine.medical_specialty, Pathology, Necrosis, Bevacizumab, coagulative necrosis, diffusion-weighted imaging, restriction-spectrum imaging, restriction-spectrum imaging (RSI), Oncology and Carcinogenesis, bevacizumab, lcsh:RC254-282, glioblastoma multiforme (GBM), necrosis, glioblastoma multiforme, Rare Diseases, Clinical Research, medicine, Spectrum imaging, Cancer, Temozolomide, business.industry, Neurosciences, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Gross Total Resection, Clinical Case Study, Brain Disorders, Brain Cancer, Coagulative necrosis, Cerebral blood volume, Oncology, Biomedical Imaging, Radiology, medicine.symptom, business, medicine.drug, Diffusion MRI
Abstract

Importance: With the increasing use of antiangiogenic agents in the treatment of high-grade gliomas, we are becoming increasingly aware of distinctive imaging findings seen in a subset of patients treated with these agents. Of particular interest is the development of regions of marked and persistent restricted diffusion. We describe a case with histopathologic validation, confirming that this region of restricted diffusion represents necrosis and not viable tumor. Observations: We present a case report of a 52-year-old man with GBM treated with temozolomide, radiation, and concurrent bevacizumab following gross total resection. The patient underwent sequential MRI's which included restriction-spectrum imaging (RSI), an advanced diffusion-weighted imaging (DWI) technique, and MR perfusion. Following surgery, the patient developed an area of restricted diffusion on RSI which became larger and more confluent over the next several months. Marked signal intensity on RSI and very low cerebral blood volume (CBV) on MR perfusion led us to favor bevacizumab-related necrosis over recurrent tumor. Subsequent histopathologic evaluation confirmed coagulative necrosis. Conclusion and Relevance: Our report increases the number of pathologically proven cases of bevacizumab-related necrosis in the literature from three to four. Furthermore, our case demonstrates this phenomenon on RSI, which has been shown to have good sensitivity to restricted diffusion. © 2013 Farid, Almeida-Freitas, White, McDonald, Muller, VandenBerg, Kesari and Dale.

Published
2013
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