Search

Your search keyword '"Core histones"' showing total 2 results
Start Over Descriptor "Core histones" Publisher escholarship, university of california
2 results on '"Core histones"'

1. Reconstitution of Chromatin by Stepwise Salt Dialysis.

Author

Cruz-Becerra, Grisel and Kadonaga, James T

Subjects
Biochemistry and Cell Biology, Biological Sciences, Genetics, Rare Diseases, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Core histones, DNA, Chromatin, Chromatin reconstitution, Salt dialysis, Micrococcal nuclease, Biological sciences, Biomedical and clinical sciences
Abstract

Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for a wide range of applications. Here, we describe simple and straightforward protocols for the reconstitution of chromatin by stepwise salt dialysis and the analysis of the chromatin by the micrococcal nuclease (MNase) digestion assay. Chromatin that is reconstituted with this method can be used for efficient homology-directed repair (HDR)-mediated gene edited with the CRISPR-Cas9 system as well as for biochemical studies of chromatin dynamics and function.

Published
2021

2. Reconstitution of Chromatin by Stepwise Salt Dialysis

Author

Grisel Cruz-Becerra and James T. Kadonaga

Subjects
Strategy and Management, 1.1 Normal biological development and functioning, Chromatin reconstitution, Industrial and Manufacturing Engineering, chemistry.chemical_compound, Rare Diseases, Micrococcal nuclease, Methods Article, Genetics, Salt dialysis, Gene, biology, Mechanical Engineering, Metals and Alloys, food and beverages, DNA, Core histones, In vitro, Chromatin, Histone, Biochemistry, chemistry, biology.protein, Generic health relevance, Dialysis (biochemistry), Function (biology)
Abstract

Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for a wide range of applications. Here, we describe simple and straightforward protocols for the reconstitution of chromatin by stepwise salt dialysis and the analysis of the chromatin by the micrococcal nuclease (MNase) digestion assay. Chromatin that is reconstituted with this method can be used for efficient homology-directed repair (HDR)-mediated gene edited with the CRISPR-Cas9 system as well as for biochemical studies of chromatin dynamics and function.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results