1. Rapid Screening of Bovine Milk Oligosaccharides in a Whey Permeate Product and Domestic Animal Milks by Accurate Mass Database and Tandem Mass Spectral Library
- Author
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Daniela Barile, Hyeyoung Lee, Don E. Otter, and Daniel J. Cuthbertson
- Subjects
0301 basic medicine ,buffalo milk ,Resolution (mass spectrometry) ,Databases, Factual ,Buffaloes ,cow milk ,tandem mass spectral library ,Oligosaccharides ,Tandem mass spectrometry ,computer.software_genre ,Article ,sheep milk ,03 medical and health sciences ,Databases ,fluids and secretions ,Engineering ,Tandem Mass Spectrometry ,Whey ,Animals ,Food science ,Sheep milk ,Factual ,Whey permeate ,chemistry.chemical_classification ,Chromatography ,Sheep ,Tandem ,Database ,Agricultural and Veterinary Sciences ,Chemistry ,Colostrum ,food and beverages ,General Chemistry ,Oligosaccharide ,bovine milk oligosaccharides ,whey permeate ,030104 developmental biology ,Milk ,Domestic animal ,Chemical Sciences ,Cattle ,General Agricultural and Biological Sciences ,computer ,Food Science - Abstract
A bovine milk oligosaccharide (BMO) library, prepared from cow colostrum, with 34 structures was generated and used to rapidly screen oligosaccharides in domestic animal milks and a whey permeate powder. The novel library was entered into a custom Personal Compound Database and Library (PCDL) and included accurate mass, retention time, and tandem mass spectra. Oligosaccharides in minute-sized samples were separated using nanoliquid chromatography (nanoLC) coupled to a high resolution and sensitive quadrupole-Time of Flight (Q-ToF) MS system. Using the PCDL, 18 oligosaccharides were found in a BMO-enriched product obtained from whey permeate processing. The usefulness of the analytical system and BMO library was further validated using milks from domestic sheep and buffaloes. Through BMO PCDL searching, 15 and 13 oligosaccharides in the BMO library were assigned in sheep and buffalo milks, respectively, thus demonstrating significant overlap between oligosaccharides in bovine (cow and buffalo) and ovine (sheep) milks. This method was shown to be an efficient, reliable, and rapid tool to identify oligosaccharide structures using automated spectral matching.
- Published
- 2016