1. The Genome Sequence of Lone Star Virus, a Highly Divergent Bunyavirus Found in the Amblyomma americanum Tick
- Author
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Samia N. Naccache, Narayanan Veeraraghavan, Barbara J. B. Johnson, Charles Y. Chiu, Brandy J. Russell, Mark A. Pilgard, Beniwende Kabre, and Andrea Swei
- Subjects
RNA viruses ,Bhanja virus ,Orthobunyavirus ,Ixodidae ,viruses ,Molecular Sequence Data ,lcsh:Medicine ,Genome, Viral ,Biology ,Arbovirus ,Microbiology ,Deep sequencing ,Vector Biology ,Amblyomma americanum ,03 medical and health sciences ,Ticks ,Genome Analysis Tools ,Viral classification ,Virology ,Chlorocebus aethiops ,medicine ,Animals ,Humans ,Genome Sequencing ,lcsh:Science ,Vero Cells ,030304 developmental biology ,Genetics ,0303 health sciences ,Multidisciplinary ,030306 microbiology ,lcsh:R ,High-Throughput Nucleotide Sequencing ,Genomics ,medicine.disease ,biology.organism_classification ,Heartland virus ,3. Good health ,Lone Star virus ,Emerging Infectious Diseases ,Phlebovirus ,Microbial Evolution ,lcsh:Q ,Viral Vectors ,HeLa Cells ,Research Article - Abstract
Viruses in the family Bunyaviridae infect a wide range of plant, insect, and animal hosts. Tick-borne bunyaviruses in the Phlebovirus genus, including Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) in China, Heartland virus (HRTV) in the United States, and Bhanja virus in Eurasia and Africa have been associated with acute febrile illness in humans. Here we sought to characterize the growth characteristics and genome of Lone Star virus (LSV), an unclassified bunyavirus originally isolated from the lone star tick Amblyomma americanum. LSV was able to infect both human (HeLa) and monkey (Vero) cells. Cytopathic effects were seen within 72 h in both cell lines; vacuolization was observed in infected Vero, but not HeLa, cells. Viral culture supernatants were examined by unbiased deep sequencing and analysis using an in-house developed rapid computational pipeline for viral discovery, which definitively identified LSV as a phlebovirus. De novo assembly of the full genome revealed that LSV is highly divergent, sharing
- Published
- 2013