1. In vitro study of H7N3 avian Influenza A viruses with truncated NS1 protein at aminoacids 99 and 126.
- Author
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May-Pech, F., Ciau-Carrillo, K. J., Ayora-Talavera, G., Castellanos-Huerta, I., González-Losa, R., Conde-Ferráez, L., Kantún-Moreno, N., Tellez-Isaias, G., and Hargis, B.
- Subjects
AVIAN influenza A virus ,VIRAL proteins - Abstract
In Mexico, the presence of highly pathogenic avian influenza AH7N3 affects the poultry industry since 2012, causing economic losses and being a subtype of concern due to its pandemic potential. Influenza viruses can block the immune response of the host by inhibiting interferons /β by effects of NS1 protein. Many studies show that truncations in NS1 protein can be the key to obtaining live attenuated vaccine candidates. Using reverse genetics and based on the H7N3 influenza full genome of a Mexican strain, recombinant viruses possessing a full-length NS1 or a truncated NS1 protein at the carboxyl-terminal at 99 or 126 amino acids were generated. All rescued viruses replicated to HA titers of 1:160 in MDCK cells. In growth kinetics assays, truncated viruses showed attenuation compared with full-length NS1 viruses in MDCK cells. The measurement of interferon mRNA production by infection assay in chicken embryo primary cell cultures, showed that truncated viruses were able to reduce IFN inhibition up to 5-fold compared to wild-type viruses. This is the first report of the rescue of recombinant viruses from full genome H7N3 subtype and the generation of two H7N3 viruses with truncated NS1 protein as potential candidates of live attenuated vaccines. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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