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The effects of chronic morphine administration on the rat cerebellum and silver stained nucleolus organizer regions in Purkinje cells were investigated by means of histological, histochemical and histometrical techniques. Thirty-two young (30-32 days of age) Wistar rats (equal numbers of both genders) were randomly divided into 2 groups, as control and morphine-treated, each having equal numbers of both genders, a total of 16 animals. The control animals were injected subcutaneously with 1mL/kg physiological saline, and the morphine-treated rats received 5 mg/kg morphine hydrochloride subcutaneously at daily intervals for 30 days. The thickness of the molecular and granular layers of the cerebellum, the diameters of the nuclei and nucleoli of cerebellar Purkinje neurones, and the number and size of the silver staining nucleolus organizer regions of the Purkinje cell nuclei were determined histomorphometrically. The morphine administration caused slight histological changes in the cerebellum. The molecular layer thickness of the cerebellum was significantly (P, Učinci kronične primjene morfina na mali mozak štakora i na srebrom obojene regije nukleolusne organizacije Purkinjeovih neurona istraživani su histološkim, histokemijskim i histometrijskim tehnikama. Trideset i dva mlada Wistar štakora (dob 30 - 32 dana), u jednakom omjeru spolova, metodom slučajnog izbora podijeljena su u dvije skupine s po 16 jedinki u svakoj skupini. Životinjama u kontrolnoj skupini potkožno je primijenjen 1 mL/kg fiziološke otopine, dok su životinje u pokusnoj skupini dobile 5 mg/kg morfin hidroklorida, potkožno, svaki dan tijekom 30 dana. Za određivanje zadebljanja molekularnih i granulacijskih slojeva maloga mozga, promjera jezgre i nukleolusa Purkinjeovih neurona u malom mozgu te broja i veličine srebrom obojenih regija nukleolusne organizacije Purkinjeovih neurona primijenjena je histomorfometrijska metoda. Davanje morfina izazvalo je blage histološke promjene u malom mozgu. Debljina molekularnog sloja maloga mozga značajno je smanjena (P