1. The hunt for origins of DNA replication in multicellular eukaryotes
- Author
-
John M. Urban, Susan A. Gerbi, Michael S. Foulk, and Cinzia Casella
- Subjects
Genetics ,0303 health sciences ,viruses ,DNA replication ,Review Article ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,Biology ,Budding yeast ,Genome ,DNA sequencing ,animal genetics article budding chromatin chromatin immunoprecipitation clinical evaluation clinical feature CpG island DNA determination *DNA replication origin DNA sequence *eukaryote eukaryote evolution gene mapping genetic association genetic transcription genome analysis histone modification *multicellular eukaryote nonhuman restriction fragment yeast 5 (2 bromovinyl) 2' deoxyuridine/ec [Endogenous Compound] dihydrofolate reductase/ec [Endogenous Compound] DNA/ec [Endogenous Compound] exonuclease/ec [Endogenous Compound] guanine quadruplex/ec [Endogenous Compound] lambda exonuclease/ec [Endogenous Compound] origin recognition complex/ec [Endogenous Compound] unclassified drug ,03 medical and health sciences ,Multicellular organism ,0302 clinical medicine ,Evolutionary biology ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
Origins of DNA replication (ORIs) occur at defined regions in the genome. Although DNA sequence defines the position of ORIs in budding yeast, the factors for ORI specification remain elusive in metazoa. Several methods have been used recently to map ORIs in metazoan genomes with the hope that features for ORI specification might emerge. These methods are reviewed here with analysis of their advantages and shortcomings. The various factors that may influence ORI selection for initiation of DNA replication are discussed.
- Published
- 2015