1. Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil
- Author
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Eric Delwart, Rafael Brustulin, Márcia Cristina Alves Brito Sayão Lobato, Shirley Vasconcelos Komninakis, Élcio Leal, Maria de Fátima Neves dos Santos Abrão, Rogério Togisaki das Chagas, Cassia Vitória de Deus Alves Soares, Xutao Deng, Adriana Luchs, Audrey Cilli, Danielle Elise Gill, Maria da Aparecida Rodrigues Teles, Flavio Augusto de Pádua Milagres, Ester Cerdeira Sabino, and Antonio Charlys da Costa
- Subjects
Microbiology (medical) ,lcsh:Arctic medicine. Tropical medicine ,Genotype ,lcsh:RC955-962 ,Short Communication ,030231 tropical medicine ,lcsh:QR1-502 ,Genome ,lcsh:Microbiology ,Sapovirus ,DNA sequencing ,Deep sequencing ,deep sequencing ,03 medical and health sciences ,0302 clinical medicine ,genotypes ,parasitic diseases ,Humans ,Typing ,Child ,Phylogeny ,Caliciviridae Infections ,Genetics ,Whole genome sequencing ,biology ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,biology.organism_classification ,Caliciviridae ,Gastroenteritis ,sapovirus ,Acute Disease ,gastroenteritis ,human activities ,Brazil - Abstract
Human sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characterization in the country precedes that of the present study. This is the first complete genome sequence of genotype GI.3 in Brazil. The data obtained in this investigation can contribute to the augmentation of the database on the molecular diversity of HSaVs strains circulating in Brazil, and to the improvement of current typing protocols.
- Published
- 2019
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