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Background & Objective: screening for the prevalence of skeletal disorders is needed for the early prevention and intervention of the disorders. For this purpose, the present study aimed to determine the prevalence of musculoskeletal disorders among surgical technologists and anesthesiologists. Materials & Methods: In this analytical - descriptive cross-sectional study, 167 personnel working in the operating room of Isfahan, Iran in 2018, were evaluated using the random sampling technique. The data from the general Nordic and Cornell questionnaires were analyzed using SPSS, version 20, software as well as the statistical tests including T-test, Chi-square, Mann-Whitney U and logistic regression. Results: Women constituted 82% of our population. The mean age and work experience of the subjects were 32.68 ± 8.64 and 10.1 ± 8.3 years, respectively. The prevalence of musculoskeletal disorders among the surgical technologists and anesthesiologists was 96.74 and 90.9, respectively. Conclusion: Given that the occupational health status of surgical technologists and anesthesiologists was not at a desirable level, this could compromise the health and work quality of the surgical team; therefore, it seems that the inclusion of some measures such as exercise, modified ergonomics` equipment, and striving to improve one's social and psychological well-being could be efficient and beneficial to prevent these disorders.