1. Increased uncoupling protein-2 and -3 gene expressions in skeletal muscle of STZ-induced diabetic rats
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Jun Oka, Daniel Ricquier, Asako Suga, Yoshio Namba, Shuji Inoue, Jean-Paul Giacobino, Takayuki Kashiwa, Toshimasa Osaka, Tsutomu Hirano, Kiyomitsu Nemoto, Haruaki Kageyama, and Misato Kashiba
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Male ,endocrine system diseases ,medicine.medical_treatment ,White adipose tissue ,Brown adipose tissue ,Biochemistry ,Ion Channels ,Rats, Sprague-Dawley ,Adipose Tissue, Brown ,Structural Biology ,Uncoupling protein ,Insulin ,Uncoupling Protein 3 ,Uncoupling Protein 2 ,Uncoupling Protein 1 ,Streptozotocin ,medicine.anatomical_structure ,Diabetic rat ,Liver ,Gastrocnemius muscle ,medicine.drug ,medicine.medical_specialty ,Biophysics ,Biology ,Streptozocin ,Diabetes Mellitus, Experimental ,Mitochondrial Proteins ,Internal medicine ,Genetics ,medicine ,Animals ,RNA, Messenger ,Muscle, Skeletal ,Molecular Biology ,Skeletal muscle ,Membrane Proteins ,Membrane Transport Proteins ,Proteins ,nutritional and metabolic diseases ,Cell Biology ,Rats ,Endocrinology ,Gene expression ,Carrier Proteins ,Thermogenesis - Abstract
Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ-diabetic rats using Northern blot. UCP-1, -2 and -3 mRNA expressions in BAT were all remarkably lower in STZ-diabetic rats than those in control rats. Both UCP-2 and -3 gene expressions in gastrocnemius muscle were substantially elevated in STZ-diabetic rats and insulin treatment restored UCP gene expressions to normal levels. These results suggest that in STZ-diabetic rats, the overexpression of UCP-2 and UCP-3 in skeletal muscle provides a defense against hypothermogenesis caused by decreased UCPs in BAT.
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