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1. Additional file 1 of Identification of molecular biomarkers associated with disease progression in the testis of bulls infected with Besnoitia besnoiti

Author

González-Barrio, David, Diezma-Díaz, Carlos, Gutiérrez-Expósito, Daniel, Tabanera, Enrique, Jiménez-Meléndez, Alejandro, Pizarro, Manuel, González-Huecas, Marta, Ferre, Ignacio, Ortega-Mora, Luis M., and Álvarez-García, Gema

Abstract

Additional file 1: Primers used for RT-PCR expression genes.

Published
2021
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2. Additional file 3 of Identification of molecular biomarkers associated with disease progression in the testis of bulls infected with Besnoitia besnoiti

Author

González-Barrio, David, Diezma-Díaz, Carlos, Gutiérrez-Expósito, Daniel, Tabanera, Enrique, Jiménez-Meléndez, Alejandro, Pizarro, Manuel, González-Huecas, Marta, Ferre, Ignacio, Ortega-Mora, Luis M., and Álvarez-García, Gema

Abstract

Additional file 3: Quantification of the immunohistochemically labelled T and B lymphocytes and phagocytic cells in the testes. Numbers represent the percentage of cell populations in each tissue analysed. Percentages are representative of three randomly selected medial tissues from each male, and cells were counted in 20 random 20 × fields of each tissue.

Published
2021
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3. Additional file 2: of The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Author

Pastor-Fernández, Iván, Regidor-Cerrillo, Javier, Álvarez-García, Gema, Marugán-Hernández, Virginia, García-Lunar, Paula, Hemphill, Andrew, and Ortega-Mora, Luis

Abstract

Clustal alignment of the up-stream regions of the NcNTPase 1, 2 and 3. Cis-acting elements (yellow) and the ORF of the NcNTPase genes (light blue) are displayed on the figure. Specific primers were designed to amplify the up-stream sequences of the NcNTPase 1, 2 and 3 as indicated in the table below. Forward primers were specific for the NcNTPase 1, 2 and 3 sequences, whilst reverse primer was common for all the copies. PCR amplification yielded a single fragment with the expected molecular weight (see figure below). (DOCX 53 kb)

Published
2016
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4. Additional file 1: of The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Author

Pastor-Fernández, Iván, Regidor-Cerrillo, Javier, Álvarez-García, Gema, Marugán-Hernández, Virginia, García-Lunar, Paula, Hemphill, Andrew, and Ortega-Mora, Luis

Abstract

Clustal alignment of the genome NcNTPase and allele sequences from GeneBank database and this study. Primers for sequencing clones 1, 2, and 3 are displayed in the table below. (DOCX 46 kb)

Published
2016
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5. Additional file 3: Figure S2. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Comparisons of tachyzoite yield for each isolate for each time point in proliferation assay (box plots). A: 24 hpi; B: 48 hpi; C: 72 hpi; D: 96 hpi; E: 120 hpi; F: 144 hpi. Means and quartiles are presented. Significant differences between the isolates (p

6. Additional file 2: Table S1. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Parameters estimated based on logistic growth model and influence of time on tachyzoite yield in proliferation assay. (DOCX 15 kb)

7. Additional file 2: Table S1. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Parameters estimated based on logistic growth model and influence of time on tachyzoite yield in proliferation assay. (DOCX 15 kb)

8. Additional file 1: Figure S1. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Comparisons of invasion events of each isolate for each time point (box plots). A: 4 hpi; B: 6 hpi; C: 8 hpi; D: 24 hpi; E: 72 hpi. Means and quartiles are presented. Significant differences between the isolates (p

9. Additional file 4: Table S2. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Parameters estimated based on exponential growth model and comparison of doubling times in proliferation assay up to 72 hpi$. (DOCX 14 kb)

10. Additional file 4: Table S2. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Parameters estimated based on exponential growth model and comparison of doubling times in proliferation assay up to 72 hpi$. (DOCX 14 kb)

11. Additional file 3: Figure S2. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Comparisons of tachyzoite yield for each isolate for each time point in proliferation assay (box plots). A: 24 hpi; B: 48 hpi; C: 72 hpi; D: 96 hpi; E: 120 hpi; F: 144 hpi. Means and quartiles are presented. Significant differences between the isolates (p

12. Additional file 1: Figure S1. of Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates

Author

Frey, Caroline, Regidor-Cerrillo, Javier, Marreros, Nelson, García-Lunar, Paula, Gutiérrez-Expósito, Daniel, Schares, Gereon, Jitender Dubey, Gentile, Arcangelo, Jacquiet, Philippe, Shkap, Varda, Cortes, Helder, Ortega-Mora, Luis, and Álvarez-García, Gema

Subjects
3. Good health
Abstract

Comparisons of invasion events of each isolate for each time point (box plots). A: 4 hpi; B: 6 hpi; C: 8 hpi; D: 24 hpi; E: 72 hpi. Means and quartiles are presented. Significant differences between the isolates (p

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