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Additional file 2: List L1. List of included studies. Table S1. Summary of characteristics of included studies.

Additional file 3: Table S2. Baseline characteristics of patients in the analysis of haematological response. Table S3. Association between haemoglobin change and baseline value. Table S4. Bi-variable analysis of factors associated with drop in haemoglobin on Day 7. Table S5. Bi-variable analysis of factors associated with anaemia on Day 7. Table S6. Univariable analysis of recovery from anaemia. Table S7. Haemoglobin measurements in patients with severe anaemia at any time. Table S8. Baseline characteristics of patients included in the analysis of adverse events. Table S9. Listing of Serious Adverse Events reported in any study. Table S10. Baseline characteristics of patients included in the analysis of haemoglobinuria. Table S11. Univariable analysis for haemoglobinuria. Table S12. Multivariable model of change in haemoglobin on Day 7 in sub-Saharan Africa. Fig. S1. Map of study sites. Fig. S2. Haemoglobin in G6PD deficient patients not included in the Day 7 analysis. Fig. S3. Relationship between haemoglobin at baseline and Day 7 as absolute change. Fig. S4. Relationship between haemoglobin at baseline and Day 7 as fractional change Fig. S5. Relationship between baseline parasitaemia and haematological endpoints. Fig. S6. Dose response relationship for haematological endpoints. Fig. S7. Time to recovery by day when anaemia was first recorded. Fig. S8. Meta-analysis of odds of haemoglobinuria after PQ administration. Fig. S9. Distribution of parasitaemia (observed) used in the simulation study. Figure S10- Predicted risk of severe anaemia (Hb

Additional file 4: Table S13. Risk of bias assessment. Table S14. Individual participant data not available. Fig. S12. Sensitivity analysis for the main haematological results.

Additional file 1. Tables S1–S5 and Figure S1.

Additional file 1: Table S2. Primers sequences, restriction enzymes and annealing temperatures of PCR-RFLP protocol used.

Additional file 1. GeoDx. An online open source tool to guide G6PD diagnostics procurement.

Additional file 2: Table S3. Details regarding SNP and amino acid changes of the seven main G6PD variants along with sequences of the PCR primers, allele-specific primers and locus-speficic probes used to test samples collected from Cambodian males, Cambodia, National Malaria Survey 2013.

Additional file 1: S1 Table. Definition of primary efficacy endpoint by initial species. S2 Table. Dose of each compound given to the participants. S3 Table. The baseline characteristics of women with Plasmodium falciparum mono-infection. S4 Table. The baseline characteristics of women with Plasmodium vivax mono-infection. S5 Table. The baseline characteristics of patients with Plasmodium falciparum infection, including both P. falciparum mono-infection and co-infection of P. falciparum and Plasmodium vivax. S6 Table. Cumulative proportion of fever and parasite clearance of falciparum and vivax for each treatment arm among pregnant women with history of fever or documented fever at enrolment. S7 Table. Description of gametocyte carriage, congenital and placental malaria. S8 Table. Univariable and multivariable analyses of the risk of PCR-corrected treatment failure in pregnant women with uncomplicated falciparum malaria using a Cox proportional hazard model. S9 Table. Univariable and multivariable analyses on the risk of PCR-corrected treatment failure in pregnant women with uncomplicated falciparum malaria using a Cox proportional hazard model with Pfkelch13 mutations. S10 Table. Prevalence of symptoms before and after treatment. S11 Table. Description of pregnancy outcomes. S12 Table. Details of pregnant women who had miscarriage or stillbirth. S13 Table. Details of congenital abnormality and ICD-10 coding. S14 Table. Details of neonatal mortality. S15 Table. Univariable and multivariable linear regression analyses of the characteristics associated with gestational week at birth among those who had malaria in pregnancy. S16 Table. Univariable and multivariable linear regression analyses of the characteristics associated with SGA z-score (birthweight for gestational age and newborn sex at birth). S17 Table. Birth outcomes in pregnant women who had malaria and were enrolled in the first trimester. S1 Figure. Monthly number of pregnant women with malaria for each malaria species over the study period (from February 2010 to August 2016). S2 Figure. Kaplan-Meier survival curves for antimalarials in pregnancy: Panel a. recurrence of any malaria species, Panel b. Plasmodium falciparum PCR-corrected efficacy censoring intercalated Plasmodium vivax malaria, Panel c. P. falciparum PCR-uncorrected efficacy , and Panel d. P. vivax parasitaemia after P. falciparum mono-infection. S3 Figure. Kaplan-Meier survival curves for parasite clearance of Plasmodium falciparum (Panel a) or Plasmodium vivax (Panel b) for each treatment arm. S4 Figure. Fractional change in haematocrit from baseline during the follow-up for each species (Panel a) or treatment stratified by malaria species (Panel b).

Additional file 2: Table S3. Details regarding SNP and amino acid changes of the seven main G6PD variants along with sequences of the PCR primers, allele-specific primers and locus-speficic probes used to test samples collected from Cambodian males, Cambodia, National Malaria Survey 2013.

Additional file 1: S1 Table. Definition of primary efficacy endpoint by initial species. S2 Table. Dose of each compound given to the participants. S3 Table. The baseline characteristics of women with Plasmodium falciparum mono-infection. S4 Table. The baseline characteristics of women with Plasmodium vivax mono-infection. S5 Table. The baseline characteristics of patients with Plasmodium falciparum infection, including both P. falciparum mono-infection and co-infection of P. falciparum and Plasmodium vivax. S6 Table. Cumulative proportion of fever and parasite clearance of falciparum and vivax for each treatment arm among pregnant women with history of fever or documented fever at enrolment. S7 Table. Description of gametocyte carriage, congenital and placental malaria. S8 Table. Univariable and multivariable analyses of the risk of PCR-corrected treatment failure in pregnant women with uncomplicated falciparum malaria using a Cox proportional hazard model. S9 Table. Univariable and multivariable analyses on the risk of PCR-corrected treatment failure in pregnant women with uncomplicated falciparum malaria using a Cox proportional hazard model with Pfkelch13 mutations. S10 Table. Prevalence of symptoms before and after treatment. S11 Table. Description of pregnancy outcomes. S12 Table. Details of pregnant women who had miscarriage or stillbirth. S13 Table. Details of congenital abnormality and ICD-10 coding. S14 Table. Details of neonatal mortality. S15 Table. Univariable and multivariable linear regression analyses of the characteristics associated with gestational week at birth among those who had malaria in pregnancy. S16 Table. Univariable and multivariable linear regression analyses of the characteristics associated with SGA z-score (birthweight for gestational age and newborn sex at birth). S17 Table. Birth outcomes in pregnant women who had malaria and were enrolled in the first trimester. S1 Figure. Monthly number of pregnant women with malaria for each malaria species over the study period (from February 2010 to August 2016). S2 Figure. Kaplan-Meier survival curves for antimalarials in pregnancy: Panel a. recurrence of any malaria species, Panel b. Plasmodium falciparum PCR-corrected efficacy censoring intercalated Plasmodium vivax malaria, Panel c. P. falciparum PCR-uncorrected efficacy , and Panel d. P. vivax parasitaemia after P. falciparum mono-infection. S3 Figure. Kaplan-Meier survival curves for parasite clearance of Plasmodium falciparum (Panel a) or Plasmodium vivax (Panel b) for each treatment arm. S4 Figure. Fractional change in haematocrit from baseline during the follow-up for each species (Panel a) or treatment stratified by malaria species (Panel b).

Additional file 3. PCR-based ligase detection reaction-fluorescent microsphere assay (PCR-LDR-FMA) protocol.

Additional file 3. PCR-based ligase detection reaction-fluorescent microsphere assay (PCR-LDR-FMA) protocol.