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1. Additional file 2 of Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep

Author

Pérez-Caballero, Raúl, Martínez-Moreno, F. Javier, Corripio-Miyar, Yolanda, McNeilly, Tom N., Cwiklinski, Krystyna, Dalton, John P., Zafra, Rafael, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro

Subjects
Data_FILES
Abstract

Additional file 2: Experiment schedule.

Published
2021
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2. Additional file 1 of Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep

Author

Pérez-Caballero, Raúl, Martínez-Moreno, F. Javier, Corripio-Miyar, Yolanda, McNeilly, Tom N., Cwiklinski, Krystyna, Dalton, John P., Zafra, Rafael, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro

Subjects
fluids and secretions, parasitic diseases, digestive system diseases
Abstract

Additional file 1: Protein expression profile of F. hepatica at different developing stages.

Published
2021
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3. Additional file 3 of Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep

Author

Pérez-Caballero, Raúl, Martínez-Moreno, F. Javier, Corripio-Miyar, Yolanda, McNeilly, Tom N., Cwiklinski, Krystyna, Dalton, John P., Zafra, Rafael, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro

Abstract

Additional file 3: Parasite burden in G5.

Published
2021
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4. Additional file 1: of Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis

Author

Pérez-Caballero, Raúl, F. Javier Martínez-Moreno, Zafra, Rafael, Molina-Hernández, Verónica, Pacheco, Isabel, M. Teresa Ruiz-Campillo, Escamilla, Alejandro, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro

Subjects
3. Good health
Abstract

Figure S1. Gating strategy for the identification of cell immunophenotypes by flow cytometric analysis. 1. Dot-plot samples (1): FSC and SSC in log scale are faced in order to exclude debris. Red square shows the leukocyte populations of interest. 2. Dot-plot (2.1) and histogram (2.2): once leukocyte populations are gatted, lineal FSC is faced to log SSC so that white cells can be shown and identified properly. The histogram is an extra support for the correct gating in leukocyte subsets. 3. Dot-plots for fluorochromes (3.1 and 3.2): according to the fluorochrome in each antibody, RPE or FITC channels are faced to log SSC, and in each dot-plot it is only shown the subset of interest which was gated in dot-plot 2. (DOCX 378 kb)

5. Additional file 1: of Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis

Author

Pérez-Caballero, Raúl, F. Javier Martínez-Moreno, Zafra, Rafael, Molina-Hernández, Verónica, Pacheco, Isabel, M. Teresa Ruiz-Campillo, Escamilla, Alejandro, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro

Subjects
3. Good health
Abstract

Figure S1. Gating strategy for the identification of cell immunophenotypes by flow cytometric analysis. 1. Dot-plot samples (1): FSC and SSC in log scale are faced in order to exclude debris. Red square shows the leukocyte populations of interest. 2. Dot-plot (2.1) and histogram (2.2): once leukocyte populations are gatted, lineal FSC is faced to log SSC so that white cells can be shown and identified properly. The histogram is an extra support for the correct gating in leukocyte subsets. 3. Dot-plots for fluorochromes (3.1 and 3.2): according to the fluorochrome in each antibody, RPE or FITC channels are faced to log SSC, and in each dot-plot it is only shown the subset of interest which was gated in dot-plot 2. (DOCX 378 kb)

6. Additional file 1 of Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach

Author

Buffoni, Leandro, Garza-Cuartero, Laura, Pérez-Caballero, Raúl, Zafra, Rafael, F. Javier Martínez-Moreno, Molina-Hernández, Verónica, Pérez, José, Martínez-Moreno, Álvaro, and Mulcahy, Grace

Subjects
3. Good health
Abstract

Additional file 1: Table S1. Individual fluke burden. Table S2. overlapping peptides of FhCL1. Text S1. Formula corrected OD. Figure S1. 3D model of FhCL1. Table S3. Level of statistical significance of peptides recognised in each group. Table S4. Non-specifically recognised peptides.

7. Additional file 1 of Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach

Author

Buffoni, Leandro, Garza-Cuartero, Laura, Pérez-Caballero, Raúl, Zafra, Rafael, F. Javier Martínez-Moreno, Molina-Hernández, Verónica, Pérez, José, Martínez-Moreno, Álvaro, and Mulcahy, Grace

Subjects
3. Good health
Abstract

Additional file 1: Table S1. Individual fluke burden. Table S2. overlapping peptides of FhCL1. Text S1. Formula corrected OD. Figure S1. 3D model of FhCL1. Table S3. Level of statistical significance of peptides recognised in each group. Table S4. Non-specifically recognised peptides.

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