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2. Additional file 1 of Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments

Author

Lison, Dominique, Saloua Ibouraadaten, Brule, Sybille Van Den, Todea, Milica, Vulpoi, Adriana, Turcu, Flaviu, Ziemann, Christina, Creutzenberg, Otto, Bonner, James C., Ameloot, Marcel, and Bové, Hannelore

Abstract

Additional file 1: Table S1. Summary of the MWCNT characteristics see Taylor-Just for details. Figure S1. FPLM detection of chemically purified MWCNT in absence or presence of fibroblasts. Femtosecond pulsed laser microscopy imaging of chemically purified MWCNT (red) deposited after 24 h in the absence (w/o, left) and presence (right) of MLg fibroblasts (green). Scale bars: 50 μm. Figure S2. FPLM detection of thermally purified MWCNT in absence or presence of fibroblasts. Femtosecond pulsed laser microscopy imaging of thermally purified MWCNT (red) deposited after 24 h in the absence (w/o, left) and presence (right) of MLg fibroblasts (green). Scale bars: 50 μm. Figure S3. FPLM detection of chemically purified MWCNT in absence or presence of macrophages. Femtosecond pulsed laser microscopy imaging of chemically purified MWCNT (red) deposited after 24 h in the absence (w/o, left) and presence (right) of THP-1 macrophages (green). Scale bars: 50 μm. Figure S4. FPLM detection of thermally purified MWCNT in absence or presence of macrophages. Femtosecond pulsed laser microscopy imaging of thermally purified MWCNT (red) deposited after 24 h in the absence (w/o, left) and presence (right) of THP-1 macrophages (green). Figure S5. Validation of the emission signal of the different MWCNTs. (A) Emission fingerprint of the MWCNTs under femtosecond pulsed laser illumination (excitation 810 nm, 80 MHz, about 10 mW laser power on the sample) confirming the white-light generation of each MWCNT. Intensity normalized to the highest value of each spectrum. For each MWCNT, the spectrum represents the mean of 9 spectra (three technical replicates of three experimental replicates). Spectra are recorded from the wells without cells. (B) Comparison of the mean ± standard deviation intensity values of the spectrum recorded between 400 and 410 nm. No significant difference among the MWCNTs (one-way ANOVA followed by Tukey’s multiple comparison test).

Published
2021
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3. Additional file 4 of The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method

Author

Taylor-Just, Alexia J., Ihrie, Mark D., Duke, Katherine S., Lee, Ho Young, You, Dorothy J., Salik Hussain, Vamsi K. Kodali, Ziemann, Christina, Creutzenberg, Otto, Vulpoi, Adriana, Turcu, Flaviu, Potara, Monica, Todea, Milica, Brule, Sybille Van Den, Lison, Dominique, and Bonner, James C.

Abstract

Additional file 4. XPS C1s High resolution spectra with the peak positions of the possible bonds. (PDF 176 kb)

Published
2020
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4. Additional file 1 of The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method

Author

Taylor-Just, Alexia J., Ihrie, Mark D., Duke, Katherine S., Lee, Ho Young, You, Dorothy J., Salik Hussain, Vamsi K. Kodali, Ziemann, Christina, Creutzenberg, Otto, Vulpoi, Adriana, Turcu, Flaviu, Potara, Monica, Todea, Milica, Brule, Sybille Van Den, Lison, Dominique, and Bonner, James C.

Abstract

Additional file 1. Raman spectra of MWCNTs samples showing the G (~ 1570 cm− 1) and D (~ 1346 cm− 1) mode.

Published
2020
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5. Additional file 2 of The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method

Author

Taylor-Just, Alexia J., Ihrie, Mark D., Duke, Katherine S., Lee, Ho Young, You, Dorothy J., Salik Hussain, Vamsi K. Kodali, Ziemann, Christina, Creutzenberg, Otto, Vulpoi, Adriana, Turcu, Flaviu, Potara, Monica, Todea, Milica, Brule, Sybille Van Den, Lison, Dominique, and Bonner, James C.

Subjects
Computer Science::Operating Systems
Abstract

Additional file 2. Curve fitting of first ordered Raman spectra.

Published
2020
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6. Additional file 3 of The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method

Author

Taylor-Just, Alexia J., Ihrie, Mark D., Duke, Katherine S., Lee, Ho Young, You, Dorothy J., Salik Hussain, Vamsi K. Kodali, Ziemann, Christina, Creutzenberg, Otto, Vulpoi, Adriana, Turcu, Flaviu, Potara, Monica, Todea, Milica, Brule, Sybille Van Den, Lison, Dominique, and Bonner, James C.

Abstract

Additional file 3. Estimation of ID1/IG ratios from Raman spectroscopy data.

Published
2020
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8. Additional file 11: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi, respiratory system, respiratory tract diseases
Abstract

CBNP did not induce oxidative stress or a pro-inflammatory response in intrapulmonary airways. (PDF 106 kb)

Published
2017
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9. Additional file 1: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Abstract

Electron microscopic images of P90 and AS-PAH. (PDF 428 kb)

Published
2017
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10. Additional file 6: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
fungi, polycyclic compounds, sense organs, eye diseases
Abstract

CBNP did not affect the viability of epithelial cells, but P90-BaP and AS-PAH induced cytokine release in vitro. (PDF 91 kb)

Published
2017
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11. Additional file 8: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
nervous system, fungi, polycyclic compounds, sense organs, eye diseases
Abstract

P90-BaP and AS-PAH increased the lung wet weights after nose-only inhalation. (PDF 67 kb)

Published
2017
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12. Additional file 5: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Abstract

Supplemental material and methods. (DOCX 31 kb)

Published
2017
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13. Additional file 17: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi, sense organs, eye diseases
Abstract

P90-9NA induced cell death at the higher concentration. (PDF 641 kb)

Published
2017
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14. Additional file 9: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
fungi, respiratory system, respiratory tract diseases
Abstract

CBNP did not increase enzyme nor total protein levels in BAL and did not induce oxidative DNA-damage in BAL cells after nose-only inhalation. (PDF 80 kb)

Published
2017
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15. Additional file 20: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Abstract

No CBNP increased ciliary beat frequency at 30 μg/ml. (PDF 5594 kb)

Published
2017
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16. Additional file 19: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, fluids and secretions, nervous system, fungi, polycyclic compounds
Abstract

AS-PAH induced mucus release and cell death. (PDF 639 kb)

Published
2017
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17. Additional file 15: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi, sense organs, eye diseases
Abstract

P90 did not cause agglomerates at 1 μg/ml, but released mucus impaired particle transport. (PDF 1144 kb)

Published
2017
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18. Additional file 18: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi, sense organs, eye diseases
Abstract

P90 induced mucus release. (PDF 1021 kb)

Published
2017
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19. Additional file 2: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Abstract

AS-PAH exhibited a less PAH content and a PAH mix compared to the coated P90 nanoparticles. (PDF 308 kb)

Published
2017
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20. Additional file 3: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Abstract

Primer pairs and probes used by quantitative real time RT-PCR. (PDF 75 kb)

Published
2017
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21. Additional file 7: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, animal diseases, fungi, respiratory system
Abstract

CBNP aerosol concentration and results of Marple impactor measurements used by nose-only inhalation experiments. (PDF 56 kb)

Published
2017
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22. Additional file 10: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi, otorhinolaryngologic diseases, respiratory system
Abstract

Only AS-PAH induced exposure-related histological alteration in lung tissue after nose-only inhalation. (PDF 72 kb)

Published
2017
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23. Additional file 16: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
nervous system, fungi, polycyclic compounds, sense organs, eye diseases
Abstract

P90-BaP induced cell death. (PDF 707 kb)

Published
2017
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24. Additional file 4: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi
Abstract

Staining of necrotic and apoptotic cells in the epithelial layer. (PDF 295 kb)

Published
2017
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25. Additional file 12: of Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

Author

Lindner, Karina, Ströbele, Michael, Schlick, Sandra, Webering, Sina, Jenckel, André, Kopf, Johannes, Danov, Olga, Sewald, Katherina, Buj, Christian, Creutzenberg, Otto, Tillmann, Thomas, Pohlmann, Gerhard, Ernst, Heinrich, Ziemann, Christina, Hüttmann, Gereon, Heine, Holger, Bockhorn, Henning, Hansen, Tanja, König, Peter, and Fehrenbach, Heinz

Subjects
body regions, nervous system, fungi, sense organs, eye diseases
Abstract

P90 agglomerates increased ciliary beat frequency and released mucus impaired particle transport speed. (PDF 573 kb)

Published
2017
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