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Figure S10. Alignment of promoter sequence of CsUP from WT and up. (PDF 108 kb)

Figure S12. Alignment of promoter sequence of Csa1G535810 from WT and up. (PDF 108 kb)

Figure S8. Genomic DNA sequence alignment of Cs535790 from WT and up (PDF 67 kb)

Figure S5. Alignment of predicted protein sequences between WT and CGN19839. The amino acid highlighted in red is the acid alternation (Ile47 in WT to Val47 in CGN19839) caused by the SNP in the first exon of Csa1G535800. The amino acids highlighted in yellow are abnormal in CGN19839 and the amino acids highlighted in blue are missing in CGN19839. The DnaJ domain at the C-terminal is underlined (PDF 81 kb)

Figure S3. Pedicel orientation of B1 and CGN19839 indicating that both inbred lines have the upward-pedicel phenotype (PDF 271 kb)

Figure S9. Genomic DNA sequence alignment of Cs535810 from WT and up (PDF 43 kb)

Figure S11. Alignment of promoter sequence of Csa1G535790 from WT and up. (PDF 109 kb)

Figure S1. Seven polymorphic markers between WT and the M DNA pool (PDF 71 kb)

Table S1. Primers used for mapping, cloning and qPCR. (PDF 186â kb) (PDF 185 kb)

Figure S7. Alignment of coding sequence of CsUP from WT and up with 19 other cucumber lines. The whole coding sequence length is 1404 bp. The 491–495 bp position of up and B1 is the 5-bp deletion highlighted in blue. The 139 bp position of CGN19839 that altered from A to G is highlighted in red, and the 1234–1237 bp position in CGN19839 is the 4-bp deletion in blue (PDF 451 kb)

Figure S2. Partial results of linkage analysis with indel-CsUp demonstrating that this marker is co-segregated with the upward-pedicel phenotype (PDF 91 kb)

Figure S4. Structure of Csa1G535800 and two mutation sites in CGN19839. a Structure of Csa1G535800. Boxes and lines indicate exons and introns, respectively. Two green boxes in Csa1G535800 indicate the 5′ UTR and the 3′ UTR. Sequencing results revealed that, compared to WT, CGN19839 has a SNP in the first exon and a 4-bp deletion in the sixth exon of Csa1G535800. The yellow box shows the deletion sequence in CGN19839. b The position of the SNP and the 4-bp deletion in the encoding sequence. Sequencing results revealed that there was a SNP (‘A’ in C-8-6 and ‘G’ in CGN19839) at 139 bp and a 4-bp deletion at 1234–1237 bp from the ATG start codon (PDF 188 kb)

Figure S6. Genomic DNA sequence alignment of CsUP from WT, up and CGN19839 (PDF 231 kb)

Figure S13. Expression level of Csa1G535790 and Csa1G535810 in pedicel at different stages. P1 pedicels from 1 to 1.5â cm length young fruits; P2 pedicels from the young fruits of the day before female flowers open; P3 pedicels from the young fruits with opening female flowers. Data are displayed as the ratio of expression to CsActin3 with three biological replicates. Error bars represent standard error (SE) (PDF 133 kb)