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Histological analysis of chondrogenic differentiation. Pellets slides were cut and stained with Alcian Blue and Red Kernechtrot (A–C) or Sirius Red (D–F) to determine proteoglycan and collagen synthesis, respectively. A semiquantitative study of the distribution of stained descriptors was processed using Image J (National Institutes of Health, Bethesda, MD, USA). A custom-written Image J program was used to measure the percentage area from the whole section of the pellet. A chromatic segmentation of each histological stain was performed using the hue, saturation, and brightness properties of the images with the «Color Thresholder ImageJ» function. (DOCX 2164 kb)

Histological analysis of chondrogenic differentiation. Pellets slides were cut and stained with Alcian Blue and Red Kernechtrot (A–C) or Sirius Red (D–F) to determine proteoglycan and collagen synthesis, respectively. A semiquantitative study of the distribution of stained descriptors was processed using Image J (National Institutes of Health, Bethesda, MD, USA). A custom-written Image J program was used to measure the percentage area from the whole section of the pellet. A chromatic segmentation of each histological stain was performed using the hue, saturation, and brightness properties of the images with the «Color Thresholder ImageJ» function. (DOCX 2164 kb)

Maternal factors. Table S2. Labor and delivery factors. Table S3 Newborn factors. Table S4. Impact of pre-thawing parameters on proliferation and chondrogenic differentiation. (DOCX 47 kb)

Maternal factors. Table S2. Labor and delivery factors. Table S3 Newborn factors. Table S4. Impact of pre-thawing parameters on proliferation and chondrogenic differentiation. (DOCX 47 kb)