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Additional file 5: Figure S4. Irinotecan resistance induced by different concentration gradients of BMI1 in CRC cells. (A) SW480 cells were transiently transfected with the indicated amounts of BMI1. The protein level of BMI1 was detected by Western blotting after 48 h. (B) Representative growth of the indicated cells as determined by a CCK8 assay. (C) The number of subpopulation cells with the CD44+/CD133+ phenotype in the indicated SW480 cells (left). Quantification of cells with the CD44+/CD133+ phenotype is shown in the histogram (right). (D) Apoptosis assay of the indicated cells by flow cytometry (left). Statistical analysis of the flow cytometry results (right). (E) Typical images from the sphere formation assay of the indicated lentivirus-infected cells treated with or without irinotecan. The error bars represent the mean ± SD from three independent experiments. **p

Additional file 3: Figure S2. Circ_001680 affected the growth ability of CRC in vitro. (A) Representative transwell images of the effect of circ_001680 on the migration of the indicated cells (left). Statistical analysis of the transwell assay results (right). (B) The wound healing assay results showing divergent migration capacities at 4 regular intervals in the indicated cells (left); the statistical analysis is shown on the right. The error bars represent the means ± SDs from three independent experiments. *p