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Additional file 2. Contains a supplementary figure with figure title and legend.

Additional file 1. Contains two supplementary tables.

Additional file 3. Contains the STROBE checklist for observational studies.

Additional file 1: Figure S1. PCA plot of β-methylation for the four sample groups, showing one outlier. The plot is coloured by different groups. Blue group did not develop MetS, but received CBCT, green did not develop MetS and did not receive CBCT, red developed MetS and received CBCT, and black developed MetS and did not receive CBCT. Figure S2. Barplot of the global average methylation per sample. Blue and green indicate whether patients had received cisplatin or not, respectively. Samples were sorted descending using their average methylation value. Figure S3. Boxplot of smoking associated CpGs for the genes AHRR and F2RL3. Never, Former and Current, refer to the smoking status as presented in Table 1. Figure S4. Q-Q plots for A) CBCT model, methylation β-value as the dependent and CBCT as the independent variable, adjusted for smoking, age, and cell count. B) MetS model, MetS as the dependent and methylation β-value as the independent variable, adjusted for CBCT, smoking, age and cell count. Figure S5. Q-Q plots for A) CBCT model, methylation β-value as the dependent and CBCT as the independent variable, adjusted for smoking, and age. B) MetS model, MetS as the dependent and methylation β-value as the independent variable, adjusted for CBCT, smoking, age, testosterone and cell count. Figure S6. Venn-diagram illustrating the overlapping number of top 2000 nominally significant CpGs between the original model, and the models with the 5 individual criteria of the MetS-diagnosis as dependent variable. Criteria is according to the National Cholesterol Education Program expert panel: Hypertension = blood pressure ≥130/85 mmHg, HDL = HDL-cholesterol 102 cm, and Glucose = fasting glucose ≥6.5 mmol/L.