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Additional file 2: Figure S2. Distribution of STLV-1 proviral loads (PVLs) in the JMs positive for STLV-1 proviral DNA. The X-axis indicates PVLs ranging from 0.01%–20%, with PVLs of 0.64%–1.28% at the maximum number of individuals. The Y-axis shows the number of individuals in each PVL group.

Additional file 1: Figure S1 Distribution of anti-STLV-1 antibody titers (ABTs) in seropositive JMs. The X-axis represents antibody titers ranging from 16–262144, with an ABT of 8192 at the maximum number of individuals. The Y-axis represents the number of individuals in each antibody titer.

Additional file 3: Figure S3 a, b and c. Alignments of the STLV-1 partial nucleotide sequences in a JM troop. Total of 12 proviral DNA-positive JMs in troop D were examined as a representative (age ranges: 2-21 years of age; sex: 4 male and 8 female) for the viral sequences of first 600 nucleotides of STLV-1 3’LTR (a) and tax exon3 (b) as well as 1447 nucleotides of entire STLV-1 env (c) by direct sequencing. The results of the alignments demonstrated only one nucleotide variation in both 3’LTR and env but not tax exon3 region for each JMs.

Additional file 3: Fig. S1. Subgroup-specific Tax molecules do not differ in the transcriptional activation of the reporter construct containing a luciferase gene under control of the NF-κB binding sequence of the IL-2Ra gene. The reporter construct containing a luciferase gene fused to five repeats of the NF-κB site of the IL-2Ra gene was independently transfected into Jurkat human T-cells with or without the Tax expression plasmid. Luciferase assays were performed 24 h after transfection. There was no difference between Tax-A and Tax-B with respect to transcriptional activity. Three independent experiments were performed. Data shown as mean ± SD, n = 3.

Additional file 2: Table S2. Primer sequences for plasmid construction.