Search

Additional file 4: Figure S3. A, B: (i) LC3b I, (ii)II and (iii) LC3bII/I densitometry analyses from western blots performed on 2 independent IPF/control cohorts, C and D: relative mRNA expression of p62 and Beclin1. E. Representative images of LC3b immunostained alveolar macrophages from IPF and control samples treated with CQ or left untreated. All graphs represent median and interquartile ranges, no significant differences observed according to Mann-Whitney test.

Additional file 2: Table S1. Primer sequences for qPCR. Table S2. Clinicopathological characteristics of the subjects included in Fig. 1b. Values are expressed as means±SD or medians with range. Table S3. Clinicopathological characteristics of the subjects included in Fig. 1f and g, Fig. 3c-d and Fig. 4a-d. Values are expressed as means±SD or medians with range. Table S4. Clinicopathological characteristics of the subjects included in Fig. 2b-d Values are expressed as means±SD or medians with range. Table S5. Clinicopathological characteristics of the subjects included in Fig. 3b. Values are expressed as means±SD or medians with range.

Additional file 3: Figure S2. Mitochondrial DNA content assessed by mtDNA/gDNA ratio measuring both ND1(A) and ND5(B) relative to HGB1 in BAL cells. No statistical difference between control (n = 10) and IPF (n = 18). Representative western blot (C) and densitometry analysis (D) of mitochondria protein TOMM20 showing no statistical difference between control (n = 5) and IPF (n = 11). TOMM20 mean fluorescence intensity/cell measured from images acquired by confocal microscopy, per patient (E) and group (F). Non significant differences are highlighted by doted lines, all other comparisons showed statistically significant differences according to Kruskal Wallis test with Dunn’s test for multiple comparisons. (G) Representative image of TOMM20 staining of alveolar macrophages from IPF patient (p1). With the exception of (E) all for all other comparisons Mann-Whitney test was used, data are represented as median with interquartile ranges.