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1. Additional file 2: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon

Author

Keillen Martins-Campos, Kuehn, Andrea, Almeida, Anne, Duarte, Ana, Vanderson Sampaio, Íria Rodriguez, Silva, Sara Da, Ríos-Velásquez, Claudia, Lima, José, Pimenta, Paulo, Bassat, Quique, Müller, Ivo, Lacerda, Marcus, Wuelton Monteiro, and Guerra, Maria Barbosa

Subjects
parasitic diseases
Abstract

Table S2. PCR cycling conditions used to detect parasites and gametocytes of Plasmodium vivax parasites. (DOC 32 kb)

Published
2018
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2. Additional file 1: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon

Author

Keillen Martins-Campos, Kuehn, Andrea, Almeida, Anne, Duarte, Ana, Vanderson Sampaio, Íria Rodriguez, Silva, Sara Da, Ríos-Velásquez, Claudia, Lima, José, Pimenta, Paulo, Bassat, Quique, Müller, Ivo, Lacerda, Marcus, Wuelton Monteiro, and Guerra, Maria Barbosa

Subjects
parasitic diseases
Abstract

Table S1. PCR setup of Plasmodium-specific qPCR (QMAL) and P. vivax specific qPCR based on detection of 18S rRNA genes and RT-qPCR detecting pvs25 transcripts. For sequences of primers and probes see [35, 38]. (DOC 31 kb)

Published
2018
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3. Additional file 3: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon

Author

Keillen Martins-Campos, Kuehn, Andrea, Almeida, Anne, Duarte, Ana, Vanderson Sampaio, Íria Rodriguez, Silva, Sara Da, Ríos-Velásquez, Claudia, Lima, José, Pimenta, Paulo, Bassat, Quique, Müller, Ivo, Lacerda, Marcus, Wuelton Monteiro, and Guerra, Maria Barbosa

Subjects
parasitic diseases
Abstract

Table S3. Data from each membrane feeding assay of Anopheles aquasalis performed with samples from symptomatic and asymptomatic individuals of Plasmodium vivax. (DOC 151 kb)

Published
2018
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4. Additional file 4: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon

Author

Keillen Martins-Campos, Kuehn, Andrea, Almeida, Anne, Duarte, Ana, Vanderson Sampaio, Íria Rodriguez, Silva, Sara Da, Ríos-Velásquez, Claudia, Lima, José, Pimenta, Paulo, Bassat, Quique, Müller, Ivo, Lacerda, Marcus, Wuelton Monteiro, and Guerra, Maria Barbosa

Abstract

Table S4. Plasmids dilutions containing the sequence of the respective PCR product were used both as assay standards and to determine the limit of detection (LoD) of each assay. Generation of the plasmids is described in [35, 38]. (DOC 29 kb)

Published
2018
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5. MOESM5 of A WD40-repeat protein unique to malaria parasites associates with adhesion protein complexes and is crucial for blood stage progeny

Author

Bohl, Andreas, Kuehn, Andrea, Simon, Nina, Ngongang, Vanesa, Spehr, Marc, Baumeister, Stefan, Przyborski, Jude, Fischer, Rainer, and Pradel, Gabriele

Subjects
animal diseases, parasitic diseases, virus diseases, chemical and pharmacologic phenomena, bacterial infections and mycoses
Abstract

Additional file 4. IFA negative and PfWLP1rp1 antibody controls.

Published
2015
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13. Additional file 1 of Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities

Author

Gruenberg, Maria, Moniz, Clara Antunes, Hofmann, Natalie E., Koepfli, Cristian, Robinson, Leanne J., Nate, Elma, Wuelton Marcelo Monteiro, Gisely Cardoso De Melo, Kuehn, Andrea, Siqueira, Andre M., Nguitragool, Wang, Bassat, Quique, Lacerda, Marcus, Jetsumon Sattabongkot, Mueller, Ivo, and Felger, Ingrid

Subjects
3. Good health
Abstract

Additional file 2. Supplementary Figures.

15. Additional file 2: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
parasitic diseases, 3. Good health
Abstract

Table S2. Reaction mixes for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR. The table describes the reagents, concentrations and template volume used in the qPCR and RT-qPCR assays. (DOCX 13 kb)

16. Additional file 3: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
parasitic diseases, 3. Good health
Abstract

Table S3. Conditions for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR assays. (DOCX 14 kb)

17. Additional file 3: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
parasitic diseases, 3. Good health
Abstract

Table S3. Conditions for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR assays. (DOCX 14 kb)

18. Additional file 1: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
parasitic diseases, 3. Good health
Abstract

Table S1. Primers and probes used for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR assays including reaction efficiency and detection limits. (DOCX 16 kb)

19. Additional file 2 of Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities

Author

Gruenberg, Maria, Moniz, Clara Antunes, Hofmann, Natalie E., Koepfli, Cristian, Robinson, Leanne J., Nate, Elma, Wuelton Marcelo Monteiro, Gisely Cardoso De Melo, Kuehn, Andrea, Siqueira, Andre M., Nguitragool, Wang, Bassat, Quique, Lacerda, Marcus, Jetsumon Sattabongkot, Mueller, Ivo, and Felger, Ingrid

Subjects
InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, ComputingMilieux_COMPUTERSANDEDUCATION, Data_FILES, ComputerApplications_COMPUTERSINOTHERSYSTEMS, 3. Good health
Abstract

Additional file 1. Supplementary Table.

20. Additional file 1: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
parasitic diseases, 3. Good health
Abstract

Table S1. Primers and probes used for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR assays including reaction efficiency and detection limits. (DOCX 16 kb)

21. MOESM5 of Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions

Author

Gruenberg, Maria, Moniz, Clara, Hofmann, Natalie, Wampfler, Rahel, Koepfli, Cristian, Mueller, Ivo, Wuelton Monteiro, Lacerda, Marcus, Gisely Melo, Kuehn, Andrea, Siqueira, Andre, and Felger, Ingrid

Subjects
3. Good health
Abstract

Additional file 5: Figure S1. Fold-difference in template copy numbers detected by molecular marker Pv-mtCOX1 versus marker Pv18S rRNA.

24. MOESM2 of Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions

Author

Gruenberg, Maria, Moniz, Clara, Hofmann, Natalie, Wampfler, Rahel, Koepfli, Cristian, Mueller, Ivo, Wuelton Monteiro, Lacerda, Marcus, Gisely Melo, Kuehn, Andrea, Siqueira, Andre, and Felger, Ingrid

Subjects
parasitic diseases, macromolecular substances, 3. Good health
Abstract

Additional file 2: Table S2. Assay conditions for P. vivax qPCR targeting cox1 (Pv-mtCOX1 assay).

27. Additional file 2 of Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities

Author

Gruenberg, Maria, Moniz, Clara Antunes, Hofmann, Natalie E., Koepfli, Cristian, Robinson, Leanne J., Nate, Elma, Wuelton Marcelo Monteiro, Gisely Cardoso De Melo, Kuehn, Andrea, Siqueira, Andre M., Nguitragool, Wang, Bassat, Quique, Lacerda, Marcus, Jetsumon Sattabongkot, Mueller, Ivo, and Felger, Ingrid

Subjects
InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, ComputingMilieux_COMPUTERSANDEDUCATION, Data_FILES, ComputerApplications_COMPUTERSINOTHERSYSTEMS, 3. Good health
Abstract

Additional file 1. Supplementary Table.

29. Additional file 4: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
3. Good health
Abstract

Table S4. Univariate analysis for predictors of P. vivax infection. (DOCX 22 kb)

31. Additional file 1 of Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities

Author

Gruenberg, Maria, Moniz, Clara Antunes, Hofmann, Natalie E., Koepfli, Cristian, Robinson, Leanne J., Nate, Elma, Wuelton Marcelo Monteiro, Gisely Cardoso De Melo, Kuehn, Andrea, Siqueira, Andre M., Nguitragool, Wang, Bassat, Quique, Lacerda, Marcus, Jetsumon Sattabongkot, Mueller, Ivo, and Felger, Ingrid

Subjects
3. Good health
Abstract

Additional file 2. Supplementary Figures.

32. Additional file 4: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
3. Good health
Abstract

Table S4. Univariate analysis for predictors of P. vivax infection. (DOCX 22 kb)

33. MOESM5 of Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions

Author

Gruenberg, Maria, Moniz, Clara, Hofmann, Natalie, Wampfler, Rahel, Koepfli, Cristian, Mueller, Ivo, Wuelton Monteiro, Lacerda, Marcus, Gisely Melo, Kuehn, Andrea, Siqueira, Andre, and Felger, Ingrid

Subjects
3. Good health
Abstract

Additional file 5: Figure S1. Fold-difference in template copy numbers detected by molecular marker Pv-mtCOX1 versus marker Pv18S rRNA.

35. Additional file 2: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Author

Almeida, Anne, Kuehn, Andrea, Castro, Arthur, Vitor-Silva, Sheila, Figueiredo, Erick, Brasil, Larissa, Brito, Marcelo, Vanderson Sampaio, Bassat, Quique, Felger, Ingrid, Wanderli Tadei, Wuelton Monteiro, Mueller, Ivo, and Lacerda, Marcus

Subjects
parasitic diseases, 3. Good health
Abstract

Table S2. Reaction mixes for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR. The table describes the reagents, concentrations and template volume used in the qPCR and RT-qPCR assays. (DOCX 13 kb)

36. MOESM2 of Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions

Author

Gruenberg, Maria, Moniz, Clara, Hofmann, Natalie, Wampfler, Rahel, Koepfli, Cristian, Mueller, Ivo, Wuelton Monteiro, Lacerda, Marcus, Gisely Melo, Kuehn, Andrea, Siqueira, Andre, and Felger, Ingrid

Subjects
parasitic diseases, macromolecular substances, 3. Good health
Abstract

Additional file 2: Table S2. Assay conditions for P. vivax qPCR targeting cox1 (Pv-mtCOX1 assay).

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