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Figure S2. Histology of tissues used in this study. Histology of normal and tumour regions obtained for this study. In each case the normal regions were 4 cm or more away from the primary tumours in the same breasts. (PDF 41755 kb)

Figure S6. Stromal regions analysed by AFM. H&E staining of the normal and tumour regions of breasts from each individual that were examined by AFM (see Fig. 4a). The black squares represent the exact regions that were analysed. (PDF 36538 kb)

Figure S1. Breast tissues used in this study. Mammograms of patients examined in this study. The regions used for analysis were visually alike in most of the patients, and are outlined - red is tumour tissue, while green is normal. (PDF 698 kb)

Figure S5. Collagen organisation in normal and tumour stroma. Picrosirius-Red-stained paraffin sections visualised in bright-field or polarised light. Samples of normal (left) and tumour (right) tissue from the same individuals are shown in each case. Ducts are outlined in black and white. (PDF 82243 kb)

Figure S4. Circadian clocks in breast tissue. Left - representative Per2::Luc traces from cultures of MECs isolated from the normal and tumour regions of patients with breast cancer. Right - normalisation of Per2::Luc activity from normal and tumour MECs. (PDF 287 kb)

Peptide frequencies for individual proteins identified in the mass spectrometry analysis. This table includes accession number, gene name and raw expression values for low and high mass spectrometry (MD) samples. (DOC 208 kb)

Breast extracellular matrix (ECM) proteomic data. This table shows the ECM proteins detected in our mass spectrometry analysis of human breast tissue, plus those identified by mass spectrometry in rat mammary tumours, and the ECM of tumours resulting from the in vivo growth of the MD-MBA-231 breast cancer line. Green boxes indicate proteins that were identified in the analyses, red boxes show proteins not identified. The differences in expression may arise because completely different samples have been used, e.g., human vs rat, and normal human breast tissue vs a cancer line model. (DOC 124 kb)

Figure S3. Cytokeratin and vimentin staining of the tissues used in this study. Cytokeratin 8 and vimentin staining of normal and tumour regions used in this study. (PDF 144000 kb)

Figure S3. Cytokeratin and vimentin staining of the tissues used in this study. Cytokeratin 8 and vimentin staining of normal and tumour regions used in this study. (PDF 144000 kb)