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Additional file 1: Table S1. Baseline information of selected gliomas.

Supplementary Materials and Methods. (DOCX 30 kb).

Figure S3. Silencing of HIF1A downregulates VEGF expression. KYSE30 and KYSE150 cells were transiently transfected with ANXA2 siRNA or control non-silencing siRNA for 48 h. a Real-time RT-PCR analysis. b Western blot analysis. GAPDH was use as a loading control. (PDF 324 kb).

Figure S5. The effect of Ser25 phosphorylation on the cellular localization of ANXA2. ESCC cells stably expressing ANXA2-shRNA were transiently transfected with pcDNA3.1-ANXA2-Y23A, pcDNA3.1-ANXA2-Y23D, or empty vector. Cellular localization of exogenously expressed ANXA2-S25D or ANXA2-S25A (green) was detected by immunofluorescence staining. DAPI was used to stain nuclei (blue). Scale bar =â 30 ÂľM. (PDF 487 kb).

Figure S2. The effect of ANXA2 knockdown on cell proliferation. KYSE30 and KYSE150 cells were transiently transfected with ANXA2 siRNA or control non-silencing siRNA. Cell viability were assessed using CCK8 assay. (PDF 234 kb).

Figure S6. The effect of ANXA2 phosphorylation on MYC mRNA expression. Real-time RT-PCR analysis of MYC mRNA expression in KYSE30 and KYSE150 cells transiently transfected with pcDNA3.1-ANXA2-Y23A or pcDNA3.1-ANXA2-Y23D for 48 h. MYC mRNA levels were normalized with the exogenously expressed ANXA2 level. (PDF 150 kb).

Figure S1. Stable knockdown of ANXA2 expression in ESCC cells. KYSE30 and KYSE150 cells were transfected with ANXA2-shRNA or control scramble shRNA and stable clone cells were selected, and then subjected to the following analyses. a Real-time RT-PCR analysis. b Western blot analysis. c Transwell assay. Representative results (left) and statistical plots (right) are shown. ***, P < 0.001. (PDF 2160 kb).