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Additional file 7: Supplementary Table 7. The top 25 hypomethylated CpG sites in salivary gland tissues of IgG4-RD patients.

Additional file 1: Supplementary Table 1. Demographic and clinical characteristics of IgG4-RD patients for genome-wide DNA methylation study.

Additional file 4: Supplementary Table 4. The top 10 hypermethylated CpG sites in CD4+ T cells of IgG4-RD patients.

Additional file 3: Supplementary Table 3. The top 10 hypomethylated CpG sites in B cells of IgG4-RD patients.

Additional file 9: Supplementary Table 9. The primers for pyrosequencing.

Additional file 2: Supplementary Table 2. The top 10 hypermethylated CpG sites in B cells of IgG4-RD patients.

Additional file 8: Supplementary Table 8. The DMRs in salivary gland tissues of IgG4-RD patients.

Additional file 5: Supplementary Table 5. The top 10 hypomethylated CpG sites in CD4+ T cells of IgG4-RD patients.

Additional file 6: Supplementary Table 6. The top 25 hypermethylated CpG sites in salivary gland tissues of IgG4-RD patients.

Additional file 2: Table S1. Baseline demographic features, clinical characteristics, and laboratory parameters of 71 treatment naïve patients with IgG4-RD.

Additional file 4: Supplementary Methods.

Additional file 1: Figure S1. Purity of CD19+ B cells and CD4+ Naïve T cells. FACS file showed CD19+ B cells (A) and CD4+ naïve T cells (B) were sorted to obtain 98% purity. Figure S2. There was no significant difference of TSLP levels between IgG4-RD patients with and without allergic history. Figure S3. Gating strategy and representative FACS plots of TSLPR and IL-7Ra in CD19+ cells of HC and IgG4-RD patients. Figure S4. There was no correlation between plasma TSLP level and other laboratory parameters. Figure S5. TSLP could not polarize Naïve T cells to Tfh directly. Figure S6. The percentage of Tfh was positively correlated with the percentages of OX40L+ on B cells in the peripheral blood of patients with IgG4-RD. Figure S7. Representative western blot showed the phosphorylation of JAK-STAT family with different time. Figure S8. Weight, imaging findings and TSLP expression of Lat mice. Figure S9. Immunohistochemistry of IgG1 in affected organs of Lat mice. Figure S10. Expressions TSLP and TSLPR in affected organs of Lat mice. Figure S11. Application of different dosage of anti-TSLP antibody Lat mice.

Additional file 1: Table S1. The Primer used in RT-PCR analysis. Table S2. Top 10 up- and down-regulated mRNAs of pSS neutrophils in this study. Table S3. Statistics and comparison of the FPKM between pSS patients (n=7) and matched healthy controls (n=6). Figure S1. Process of obtaining the PBMCs and neutrophils from pSS patients and matched healthy controls (Created with BioRender.com ). Figure S2. RT-qPCR results for type I related mRNAs in pSS and healthy neutrophils (pSS=18, HC=17). (P-Value > 0.05 indicated no statistically significant difference (ns), *P-Value < 0.05, **P-Value < 0.01, ***P-Value

Additional file 1.