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Additional file 12: Figure S10. Regulation of genes of TGF-beta signaling pathway during senescence induction in MRC-5 strains. Genes of the “TGF-beta signaling” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in MRC-5 fibroblast strains. Blue color signifies genes which are commonly down-regulated during both, irradiation induced and replicative senescence.

Additional file 2: Figure S2. Heatmap showing the intersection of the most differentially expressed genes in each of the fibroblast strains (irradiated versus controls). Heatmap illustrating the log2 fold change of gene expression when comparing irradiated HFF versus controls (upper part) and irradiated MRC-5 versus controls (lower part) respectively. The horizontal axis displays the genes selected for this comparison. Genes were selected by intersecting the 200 most differentially regulated genes for each condition. This intersection contains 46 genes. The color key (top left) relates heatmap color to log2 fold change. Red color indicates a negative log2 fold change, i.e. a down-regulation under the second condition compared to the first condition, while the yellow color indicates a positive log2 fold change, i.e. an up-regulation under the second condition relative to the first condition. The dendro- gram on top of the plot clusters the genes into groups with similar expression levels for both comparisons. While most of the genes show similar log2 fold changes for both comparisons, some genes are up-regulated for one comparison, and down-regulated for the other comparison (CTGF, COLEC12, KRT19 in the middle of the plot).

Additional file 7: Figure S7. Regulation of genes of PPAR signaling pathway during senescence induction in HFF strains. Genes of the “PPAR signaling” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in HFF strains. Orange and blue colors signify genes which are commonly up- (orange) and down-regulated (blue) during both, irradiation induced and replicative senescence.

Additional file 6: Figure S6. Regulation of genes of ECM receptor interaction pathway during senescence induction in HFF strains. Genes of the “ECM receptor interaction” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in HFF strains. Orange and blue colors signify genes which are commonly up- (orange) and down-regulated (blue) during both, irradiation induced and replicative senescence.

Additional file 4: Figure S4. Regulation of genes of Cell adhesion pathway during senescence induction in HFF strains. Genes of the “Cell adhesion” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in HFF strains. Orange and blue colors signify genes which are commonly up- (orange) and down-regulated (blue) during both, irradiation induced and replicative senescence.

Additional file 5: Figure S5. Regulation of genes of Dilated cardiomyopathy pathway during senescence induction in HFF strains. Genes of the “Dilated cardiomyopathy” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in HFF strains. Orange color signifies genes which are commonly up-regulated during both, irradiation induced and replicative senescence.

Additional file 8: Figure S8. Regulation of genes of Long term depression pathway during senescence induction in HFF strains. Genes of the “Long term depression” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in HFF strains. Orange color signifies genes which are commonly up- regulated during both, irradiation induced and replicative senescence.

Additional file 11: Figure S9. Regulation of genes of NOD-like receptor signaling pathway during senescence induction in MRC-5 strains. Genes of the “NOD-like receptor signaling” pathway which are significantly down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in MRC-5 fibroblast strains. Blue color signifies genes which are commonly down-regulated during both, irradiation induced and replicative senescence.

Growth curve and percentage of SA β-Gal positive cells in WI-38 fibroblasts +/− rotenone treatment. (A) Growth curve of WI-38 fibroblasts supplemented with 0.1 μM rotenone (green), compared to DMSO-treated controls (black). (B) Percentage of SA β-Gal positive cells in 0.1 μM rotenone-treated WI-38 fibroblasts (green), compared to DMSO-treated controls (black). The bars indicate the mean ± S.D. n = 3. (DOCX 26 kb)

Up-regulated pathways in human foreskin fibroblasts on low dose rotenone treatment. Pathways significantly (p

Down-regulated pathways in human foreskin fibroblasts on low dose rotenone treatment. Pathways significantly (p

The most significant commonly up- and down-regulated genes in primary human fibroblast strains independent of their cell origin. The common genes most significantly differentially regulated at transcript levels in low dose rotenone treated MRC-5 (PDs 42, 48) and HFFs (PDs 26, 30, 34) compared to untreated controls. These genes were selected according to the statistical stringency criteria of p

Common significantly up- and down-regulated pathways in primary human fibroblast strains independent of their cell origin. Pathways significantly (p

Apoptosis induction by higher concentrations of rotenone treatment in MRC-5 fibroblasts. Numbers of days MRC-5 fibroblasts were maintained in culture before induction of apoptosis (as detected by phosphorylation of p38) in response to different concentrations of rotenone. (DOCX 15 kb)

The most significant commonly up- and down-regulated genes in primary human fibroblast strains independent of their cell origin. The common genes most significantly differentially regulated at transcript levels in low dose rotenone treated MRC-5 (PDs 42, 48) and HFFs (PDs 26, 30, 34) compared to untreated controls. These genes were selected according to the statistical stringency criteria of p

Up-regulated pathways in human foreskin fibroblasts on low dose rotenone treatment. Pathways significantly (p

Down-regulated pathways in human foreskin fibroblasts on low dose rotenone treatment. Pathways significantly (p