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Primary antibody controls for Fig. 1. As a control for specific primary antibody binding, immunostaining was performed in the absence of primary antibodies, with only fluorophore tagged secondary antibody present. We confirm no florescence signal was detected these controls for eNOS and CAV-1. (a) Detection of nuclear staining by Dapi and (b) immunostaining performed without mouse monoclonal anti-eNOS antibody but with an anti-mouse IgG secondary antibody conjugated with Alexa 488. (c) Detection of nuclear staining by Dapi and (d) immunostaining performed without rabbit polyclonal anti-CAV-1 antibody but with an anti-rabbit IgG secondary antibody conjugated with Alexa 555. (e) Detection of nuclear staining by Dapi and (d) fluorescence detection with no mouse monoclonal anti-eNOS antibody with an anti-mouse IgG secondary antibody conjugated with Alexa 555. (f) Detection of nuclear staining by Dapi and (g) fluorescence detection with no rabbit polyclonal anti-CAV-1 antibody with an anti-rabbit IgG secondary antibody conjugated with Alexa 488. (PPT 182 kb)

Primary antibody controls for Fig. 11. As a control for specific β-catenin primary antibody binding, immunostaining was carried out in the absence of the primary antibody with no florescence signal detected. (a) Detection of nuclear staining by Dapi and (b) fluorescence detection without rabbit monoclonal anti-β-catenin antibody with an anti-mouse IgG secondary antibody conjugated with Alexa 488. (PPT 102 kb)