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Additional file 6: Figure S5. Three-dimension PCA for RNAseq correlation. Principal Component coordinates were calculated using sample read counts. Figure S6 - Hierarchical clustering map for differential expression genes.

Additional file 3: Figure S3. Flowchart of sequencing and de novo assembly of A.donax leaf and root transcriptome under cadmium treatment.

Additional file 4: Figure S4. Length distribution of transcripts and unigenes.

Additional file 2: Figure S2. Effect of cadmium treatment on morpho-biometric and physiological parameters of A. donax G10 ecotype. A) Main stem height per pot. B) Biomass dry weight. C) Net photosynthesis efficiency.

Figure S6. Phylogenetic relationship among A. donax salt responsive clusters and orthologues belonging to different plant sources. a cluster 14,027–155,903 homolog of Phramites australis Na+/H+ antiporter (NHX1). b cluster 14,027–181,583 homolog of Arabidopsis thaliana Na+/H+ exchanger 2 (NHX2). c cluster 14,027–182,899 homolog of Oryza sativa CBL-interacting protein kinase 1 (CIPK1-SOS2-like). d cluster 14,027–182,899 homolog of Oryza sativa CBL-interacting protein kinase 24 (SOS2). e cluster 14,027–54,233 homolog of Setaria italica cation transporter (HKT9). f cluster 14,027–231,386 homolog of Arabidopsis thaliana persulfide dioxygenase (ETHE1). (DOCX 171 kb)

Figure S1. Effect of salt stress upon G2, G18 and G20 ecotype morpho-biometric and physiological parameters. . a Leaf number per pot. b Stem number per pot. c Main stem height. d SPAD. e Net photosynthesis. f Dry biomass (PDF 97 kb)

Table S2. Distribution of KEGG pathways for DEGs in the three sample sets. (DOCX 15 kb)